multiple freeze/thaw cycles.
Figure 1.
SDS-PAGE Gel of Typical Lot
70–95% (densitometry)
p 18INK4C43
72
95
130
170
56
34
26
References
1. Ravichandran, N. et al., Structure-based
multiple freeze/thaw cycles.
Figure 1.
SDS-PAGE Gel of Typical Lot
70–95% (densitometry)
PTPN12
34
43
72
95
130
170
56
Figure 2.
Specific Activity of Typical Lot
2,520–3,410
handling
and multiple freeze/thaw cycles.
Figure 1.
SDS-PAGE Gel of Typical Lot
70–95% (densitometry)
43
72
95
130
170
56
PTPRF
34
26
17
Figure 2.
Specific Activity of Typical
is necessary for the execution of the muscle
program. Molec. Cell, 4, 725-734 (1999).
RC,MAM 11/11-1
PCAF
34
43
72
95
130
170
56
26
2011 Sigma-Aldrich Co. LLC. All rights reserved
315 959 001
100 ml, 4 x 25 ml 03 315 843 001
SuRE/Cut Buffers SuRE/Cut Buffer A, 5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x
volume each
11 585 550 910
Taq DNA Polymerase, 1 U/µl 250 U, 1 U/μl
200 reactions in a final volume of 50 μl
11 647 679 001
1,000 U, 4 x 250 U
800 reactions in a
2 min denaturation
at 95°C.
B: without treatment
Lanes 1, 7, 13: DNA molecular weight marker VI
Lanes 2, 8: 1 ng DNA
Lanes 3, 9: 10 pg DNA
Lanes 4, 10: 0.1 pg DNA
Lanes 5, 11: 1 fg DNA
Lanes 6, 12: control
60 minutes ZFF 23 °C Yes
3 30 minutes 70% Alcohol 23 °C No
4 30 minutes 80% Alcohol 23 °C No
5 30 minutes 95% Alcohol 23 °C No
6 30 minutes 95% Alcohol 23 °C No
7 60 minutes 100% Alcohol 23 °C
Recent advances in amyotrophic
lateral sclerosis. Current Opinion in Neurology,
13(4), 397–405 (2000).
RC,MAM 11/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark
HePTP. Oncogene, 19, 858-869
(2000).
BIOMOL Green is a trademark of Enzo Life Sciences.
FF,MAM 11/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼150 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 95–129 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
kinase.
Nature, 411, 102-107 (2001).
BIOMOL Green is a trademark of Enzo Life Sciences.
FF,MAM 11/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich