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Stijn Aaron den Daas et al.
STAR protocols, 3(2), 101365-101365 (2022-05-12)
Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage
Measurement of free IGF-I saliva levels: perspectives in the detection of GH/IGF axis in athletes
Antonelli G, et al.
Clinical Biochemistry, 40(8), 545-550 (2007)
Gervasio A Lamas et al.
American heart journal, 168(1), 37-44 (2014-06-24)
Disodium ethylenediaminetetraacetic acid (EDTA) reduced adverse cardiac outcomes in a factorial trial also testing oral vitamins. This report describes the intent-to-treat comparison of the 4 factorial groups overall and in patients with diabetes. This was a double-blind, placebo-controlled, 2 ×
Luminita David et al.
Colloids and surfaces. B, Biointerfaces, 122, 767-777 (2014-09-02)
This research aimed at reporting the synthesis, characterization and evaluation of the anti-inflammatory effects of some new biomaterials based on silver nanoparticles and polyphenols rich natural extracts. A fast and eco-friendly extracellular biosynthesis of silver nanoparticles (AgNPs), using European black
Salah Al Zadjali et al.
International journal of clinical and experimental medicine, 8(5), 7160-7167 (2015-07-30)
Lead is a widely-spread environmental pollutant and a commonly-used industrial chemical that can cause multisystemic adverse health effects. However, the effects of lead exposure on diabetic animals have not been reported so far. The aim of this study is to
Conazole fungicides inhibit Leydig cell testosterone secretion and androgen receptor activation in vitro.
Roelofs MJE, et al.
Toxicology Reports, 1, 271-283 (2014)
Jim Reed et al.
Bio-protocol, 10(9), e3607-e3607 (2021-03-05)
Recognition of antigens by lymphocytes (B, T, and NK) on the surface of an antigen-presenting cell (APC) leads to lymphocyte activation and the formation of an immunological synapse between the lymphocyte and the APC. At the immunological synapse APC membrane
Karolina Borankova et al.
Cell death & disease, 14(11), 747-747 (2023-11-17)
Mitochondria are central for cancer responses to therapy-induced stress signals. Refractory tumors often show attenuated sensitivity to apoptotic signaling, yet clinically relevant molecular actors to target mitochondria-mediated resistance remain elusive. Here, we show that MYC-driven neuroblastoma cells rely on intact
Anja Täuber et al.
International journal of pharmaceutics, 489(1-2), 73-82 (2015-04-22)
Fungal infections of skin and/or nails are common diseases resulting in major challenges in topical treatment. Therefore, the objective of the present study was to develop a dermal formulation targeting both tinea pedis and onychomycosis. The antifungal agent ciclopirox olamine
Philip Randall et al.
Food and nutrition bulletin, 33(4 Suppl), S344-S359 (2013-03-01)
Wheat and maize flour fortification is a preventive food-based approach to improve the micronutrient status of populations. In 2009, the World Health Organization (WHO) released recommendations for such fortification, with guidelines on the addition levels for iron, folic acid, vitamin
Aditya Pandey et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 92(4), 269-278 (2014-06-20)
G-protein coupled receptors (GPCRs) are inherently dynamic membrane protein modulators of various important cellular signaling cascades. The apelin receptor (AR or APJ) is a class A GPCR involved in numerous physiological processes, implicated in angiogenesis during tumour formation and as
Eike Floettmann et al.
The Journal of pharmacology and experimental therapeutics, 361(2), 280-291 (2017-03-25)
Opioid-induced constipation (OIC) is a common side effect of opioid pharmacotherapy for the management of pain because opioid agonists bind to µ-opioid receptors in the enteric nervous system (ENS). Naloxegol, a polyethylene glycol derivative of naloxol, which is a derivative
Fangyang Wang et al.
The Journal of clinical investigation, 129(4), 1626-1640 (2019-02-06)
The discovery of recurrent mutations in subunits of the vacuolar-type H+-translocating ATPase (v-ATPase) in follicular lymphoma (FL) highlights a role for the amino acid- and energy-sensing pathway to mTOR in the pathogenesis of this disease. Here, through the use of
Marlies De Boeck et al.
Mutation research. Genetic toxicology and environmental mutagenesis, 786-788, 151-157 (2015-07-28)
As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiated international validation study of in vivo rat alkaline comet assay (comet assay), p-phenylenediamine dihydrochloride (PPD), o-phenylphenol sodium salt (OPP), and 2,4-diaminotoluene (2,4-DAT), were analyzed in this laboratory
The cholinergic anti-inflammatory pathway inhibits inflammation without lymphocyte relay.
Simon, et al.
Frontiers in Neuroscience, 17, 1125492-1125492 (2023)
Lijuan Zhou et al.
Current protocols in plant biology, 5(4), e20119-e20119 (2020-09-26)
Protein S-acylation, predominately in the form of palmitoylation, is a reversible lipid post-translational modification on cysteines that plays important roles in protein localization, trafficking, activity, and complex assembly. The functions and regulatory mechanisms of S-acylation have been extensively studied in
Lee-Chung Lin et al.
Methods in molecular biology (Clifton, N.J.), 1056, 63-77 (2013-12-07)
Conventional mutant screening in forward genetics research is indispensible to understand the biological operation behind any given phenotype. However, several issues, such as functional redundancy and lethality or sterility resulting from null mutations, frequently impede the functional characterization of genetic
Jonathan D Louie et al.
iScience, 27(5), 109600-109600 (2024-04-23)
Horizontal basal cells (HBCs) mediate olfactory epithelium (OE) regeneration following severe tissue injury. The dynamism of the post-injury environment is well illustrated by in silico modeling of RNA sequencing data that demonstrate an evolving HBC transcriptome. Unfortunately, spatiotemporally dynamic processes
Mary K Sherwood et al.
Chemosphere, 113, 56-61 (2014-07-30)
Modified Fenton (MF) chemistry was tested in the laboratory to treat three diesel fuel-contaminated soils from the Canadian arctic rich in soil organic matter (SOM) and Fe oxides. Reactors were dosed with hydrogen peroxide (HP), and treatment was compared in
Santiago González-López et al.
The journal of adhesive dentistry, 15(5), 481-489 (2013-04-18)
To compare the percentage of collagen exposed in dentin root thirds after two irrigation protocols with manual or rotary instrumentation using two methacrylate resin-based sealers. Forty-eight single-root human teeth were prepared with manual (n = 24) or nickeltitanium ProFile rotary
Mohd Baasir Gaisawat et al.
Nutrients, 11(9) (2019-08-29)
Probiotic supplementation plays a key role in maintaining intestinal homeostasis due to its ability to modulate gut microbiota. Although their potential as potent antioxidants have previously been explored, their ability to affect the redox status in the gut lumen of
Cheng-Chung Liu et al.
Environmental pollution (Barking, Essex : 1987), 178, 97-101 (2013-04-03)
Soil washing using a strong chelating agent is a common practice for restoring contaminated soils, but significant soil fertility degradation and high operation costs are the major disadvantages. Washing soil with a dissolved organic matter (DOM) solution has been identified
David E Moody et al.
Journal of analytical toxicology, 39(5), 374-386 (2015-04-15)
Opioid-related mortality rates have escalated. Drug interactions may increase blood concentrations of the opioid. We therefore used human liver microsomes (HLMs) and cDNA-expressed human cytochrome P450s (rCYPs) to study in vitro inhibition of buprenorphine metabolism to norbuprenorphine (CYP3A4 and 2C8)
E Holen et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 31(6), 952-964 (2001-06-26)
Chicken ovomucoid (OM, Gal d 1) has an important role in the pathogenesis of IgE-mediated allergic reactions to hen's egg white. The purpose of this study was to clarify the mechanisms of T cell recognition of ovomucoid using intact OM
Balmiki Ray et al.
Current protocols in neuroscience, Chapter 7, Unit 7-Unit 7 (2012-07-04)
Acetylcholine (ACh) is the neurotransmitter used by cholinergic neurons at the neuromuscular junction, in parasympathetic peripheral nerve terminals, and in important memory-related circuits in the brain, and takes part in other critical functions. ACh is synthesized from choline and acetyl
Q Chu et al.
Cell proliferation, 46(3), 254-262 (2013-05-23)
Human CAP10-like protein 46 kDa (hCLP46), also known as Poglut1, has been shown to be an essential regulator of Notch signalling. hCLP46 is overexpressed in primary acute myelogenous leukaemia, T-acute lymphoblastic leukaemia samples and other leukaemia cell lines. However, effects
Xiang Gao et al.
Acta radiologica (Stockholm, Sweden : 1987), 55(3), 328-334 (2013-08-22)
Previous studies have demonstrated that magnetic resonance imaging may be a method of choice to visualize transplanted pancreatic islets. However, contrast agents may interfere with microcirculation and affect graft function. To evaluate the effects manganese-containing contrast media on regional blood
Isolation of plasma membrane-associated membranes from rat liver
Suski JM, et al.
Nature Protocols, 9(2), 312-312 (2014)
Hongping Wan et al.
Colloids and surfaces. B, Biointerfaces, 193, 110977-110977 (2020-05-15)
Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function. Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage
G Lima-Oliveira et al.
British journal of biomedical science, 70(1), 6-9 (2013-04-27)
This study assesses the use of different dry K2 (dipotassium) EDTA vacuum tubes and whether or not they might represent a bias in haematological testing. Blood was collected in three dipotassium EDTA vacuum tubes from different manufacturers: Venosafe, Vacuette and
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