ED2SS

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Reclamation of copper-contaminated soil using EDTA or citric acid coupled with dissolved organic matter solution extracted from distillery sludge.

Cheng-Chung Liu et al.

Environmental pollution (Barking, Essex : 1987), 178, 97-101 (2013-04-03)

Soil washing using a strong chelating agent is a common practice for restoring contaminated soils, but significant soil fertility degradation and high operation costs are the major disadvantages. Washing soil with a dissolved organic matter (DOM) solution has been identified

Endosymbiotic and host proteases in the digestive tract of the invasive snail Pomacea canaliculata: diversity, origin and characterization.

Martín S Godoy et al.

PloS one, 8(6), e66689-e66689 (2013-07-03)

Digestive proteases of the digestive tract of the apple snail Pomacea canaliculata were studied. Luminal protease activity was found in the crop, the style sac and the coiled gut and was significantly higher in the coiled gut. Several protease bands

Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein.

Azumi Hirata et al.

BMC biotechnology, 13, 19-19 (2013-03-02)

Tk-SP is a member of subtilisin-like serine proteases from a hyperthermophilic archaeon Thermococcus kodakarensis. It has been known that the hyper-stable protease, Tk-SP, could exhibit enzymatic activity even at high temperature and in the presence of chemical denaturants. In this

Production of cloned mice by somatic cell nuclear transfer.

Satoshi Kishigami et al.

Nature protocols, 1(1), 125-138 (2007-04-05)

Although it has now been 10 years since the first cloned mammals were generated from somatic cells using nuclear transfer (NT), the success rate for producing live offspring by cloning remains < 5%. Nevertheless, the techniques have potential as important

Effective estimation of correct platelet counts in pseudothrombocytopenia using an alternative anticoagulant based on magnesium salt.

Peter Schuff-Werner et al.

British journal of haematology, 162(5), 684-692 (2013-07-03)

Pseudothrombocytopenia remains a challenge in the haematological laboratory. The pre-analytical problem that platelets tend to easily aggregate in vitro, giving rise to lower platelet counts, has been known since ethylenediamine-tetra acetic acid EDTA and automated platelet counting procedures were introduced

Paramyxovirus infections in ex vivo lung slice cultures of different host species.

D Tien Nguyen et al.

Journal of virological methods, 193(1), 159-165 (2013-06-20)

Paramyxoviruses, including measles virus (MV), human metapneumovirus (HMPV), human respiratory syncytial virus (HRSV) and canine distemper virus (CDV), are transmitted via the respiratory route. Despite their close phylogenetic relationship, the pathogenesis of these viruses is very different. To study viral

High-Throughput Cell-Free Screening of Eukaryotic Membrane Proteins in Lipidic Mimetics.

Renato Bruni

Current protocols, 2(8), e510-e510 (2022-08-05)

Membrane proteins (MPs) carry out important functions in the metabolism of cells, such as the detection of extracellular activities and the transport of small molecules across the plasma and organelle membranes. Expression of MPs for biochemical, biophysical, and structural analysis

Cell-Intrinsic Determinants of Ibrutinib-Induced Apoptosis in Chronic Lymphocytic Leukemia.

Nisar A Amin et al.

Clinical cancer research : an official journal of the American Association for Cancer Research, 23(4), 1049-1059 (2016-08-19)

Purpose: Ibrutinib, a Bruton tyrosine kinase (BTK) inhibitor, is approved for the treatment of relapsed chronic lymphocytic leukemia (CLL) and CLL with del17p. Mechanistically, ibrutinib interferes with B-cell receptor (BCR) signaling as well as multiple CLL cell-to-microenvironment interactions. Given the

Regulation of the corpus luteum by protein kinase C. I. Phosphorylation activity and steroidogenic action in large and small ovine luteal cells.

M C Wiltbank et al.

Biology of reproduction, 40(6), 1194-1200 (1989-06-01)

The activity and steroidogenic action of protein kinase C were evaluated in small and large steroidogenic ovine luteal cells. Protein kinase C activity (per mg protein) was threefold greater in large than in small luteal cells, whereas protein kinase A

Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner.

Q Chu et al.

Cell proliferation, 46(3), 254-262 (2013-05-23)

Human CAP10-like protein 46 kDa (hCLP46), also known as Poglut1, has been shown to be an essential regulator of Notch signalling. hCLP46 is overexpressed in primary acute myelogenous leukaemia, T-acute lymphoblastic leukaemia samples and other leukaemia cell lines. However, effects

Structure of MlaFB uncovers novel mechanisms of ABC transporter regulation.

Ljuvica R Kolich et al.

eLife, 9 (2020-07-01)

ABC transporters facilitate the movement of diverse molecules across cellular membranes, but how their activity is regulated post-translationally is not well understood. Here we report the crystal structure of MlaFB from E. coli, the cytoplasmic portion of the larger MlaFEDB

Monitoring the effects of chelating agents and electrical fields on active forms of Pb and Zn in contaminated soil.

Iman Tahmasbian et al.

Environmental monitoring and assessment, 185(11), 8847-8860 (2013-05-21)

The application of electrical fields and chelating agents is an innovative hybrid technology used for the decontamination of soil polluted by heavy metals. The effects of four center-oriented electrical fields and chelating agents on active fractions of lead and zinc

Doxorubicin-loaded polysaccharide nanoparticles suppress the growth of murine colorectal carcinoma and inhibit the metastasis of murine mammary carcinoma in rodent models.

Mingqiang Li et al.

Biomaterials, 51, 161-172 (2015-03-17)

As a synergistic drug combination, doxorubicin-loaded cisplatin crosslinked polysaccharide-based nanoparticles (Dex-SA-DOX-CDDP) have demonstrated enhanced antitumor efficacy and reduced systemic toxicity via optimized biodistribution, controlled drug release, prolonged blood circulation, and improved tolerability, compared to the non-crosslinked nanoparticles or free doxorubicin.

Single nucleotide polymorphisms in premature ovarian failure-associated genes in a Chinese Hui population.

Lili Ma et al.

Molecular medicine reports, 12(2), 2529-2538 (2015-05-09)

Premature ovarian failure (POF) is an ovarian defect characterized by the premature depletion of ovarian follicles in individuals <40 years old, and is a major cause of infertility in females. Genetic factors are considered to be responsible for the development

Impact of cadmium, cobalt and nickel on sequence-specific DNA binding of p63 and p73 in vitro and in cells.

Matej Adámik et al.

Biochemical and biophysical research communications, 456(1), 29-34 (2014-12-03)

Site-specific DNA recognition and binding activity belong to common attributes of all three members of tumor suppressor p53 family proteins: p53, p63 and p73. It was previously shown that heavy metals can affect p53 conformation, sequence-specific binding and suppress p53

Vtc5 Is Localized to the Vacuole Membrane by the Conserved AP-3 Complex to Regulate Polyphosphate Synthesis in Budding Yeast.

Amanda Bentley-DeSousa et al.

mBio, 12(5), e0099421-e0099421 (2021-09-22)

Polyphosphates (polyP) are energy-rich polymers of inorganic phosphates assembled into chains ranging from 3 residues to thousands of residues in length. They are thought to exist in all cells on earth and play roles in an eclectic mix of functions

Developmental validation of the PowerPlex(®) Fusion 6C System.

Martin G Ensenberger et al.

Forensic science international. Genetics, 21, 134-144 (2016-01-17)

The PowerPlex(®) Fusion 6C System is a 27-locus, six-dye, multiplex that includes all markers in the expanded CODIS core loci and increases overlap with STR database standards throughout the world. Additionally, it contains two, rapidly mutating, Y-STRs and is capable

Gestational and lactational exposure to atrazine via the drinking water causes specific behavioral deficits and selectively alters monoaminergic systems in C57BL/6 mouse dams, juvenile and adult offspring.

Zhoumeng Lin et al.

Toxicological sciences : an official journal of the Society of Toxicology, 141(1), 90-102 (2014-06-11)

Atrazine (ATR) is one of the most frequently detected pesticides in the U.S. water supply. This study aimed to investigate neurobehavioral and neurochemical effects of ATR in C57BL/6 mouse offspring and dams exposed to a relatively low (3 mg/l, estimated

A permeability assay for mouse intestinal organoids.

Stijn Aaron den Daas et al.

STAR protocols, 3(2), 101365-101365 (2022-05-12)

Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage

Brand of dipotassium EDTA vacuum tube as a new source of pre-analytical variability in routine haematology testing.

G Lima-Oliveira et al.

British journal of biomedical science, 70(1), 6-9 (2013-04-27)

This study assesses the use of different dry K2 (dipotassium) EDTA vacuum tubes and whether or not they might represent a bias in haematological testing. Blood was collected in three dipotassium EDTA vacuum tubes from different manufacturers: Venosafe, Vacuette and

Measurement of free IGF-I saliva levels: perspectives in the detection of GH/IGF axis in athletes

Antonelli G, et al.

Clinical Biochemistry, 40(8), 545-550 (2007)

Effects of Mn-DPDP and manganese chloride on hemodynamics and glucose tolerance in anesthetized rats.

Xiang Gao et al.

Acta radiologica (Stockholm, Sweden : 1987), 55(3), 328-334 (2013-08-22)

Previous studies have demonstrated that magnetic resonance imaging may be a method of choice to visualize transplanted pancreatic islets. However, contrast agents may interfere with microcirculation and affect graft function. To evaluate the effects manganese-containing contrast media on regional blood

Protein-protein interactions in the Mla lipid transport system probed by computational structure prediction and deep mutational scanning.

Mark R MacRae et al.

The Journal of biological chemistry, 299(6), 104744-104744 (2023-04-27)

The outer membrane (OM) of Gram-negative bacteria is an asymmetric bilayer that protects the cell from external stressors, such as antibiotics. The Mla transport system is implicated in the Maintenance of OM Lipid Asymmetry by mediating retrograde phospholipid transport across

Lysozyme/EDTA disodium salt and modified-atmosphere packaging to prolong the shelf life of burrata cheese

Conte A, et al.

Journal of Dairy Science, 94(11), 5289-5297 (2011)

Isolation of plasma membrane-associated membranes from rat liver

Suski JM, et al.

Nature Protocols, 9(2), 312-312 (2014)

A bioinspired mucoadhesive restores lubrication of degraded cartilage through reestablishment of lamina splendens.

Hongping Wan et al.

Colloids and surfaces. B, Biointerfaces, 193, 110977-110977 (2020-05-15)

Adsorbed lubricious films composed of biomacromolecules are natively present at all articulating interfaces in the human body where they provide ultralow friction and maintain normal physiological function. Biolubrication gets impaired due to diseases such as osteoarthritis, in which cartilage damage

Azole antifungal inhibition of buprenorphine, methadone and oxycodone in vitro metabolism.

David E Moody et al.

Journal of analytical toxicology, 39(5), 374-386 (2015-04-15)

Opioid-related mortality rates have escalated. Drug interactions may increase blood concentrations of the opioid. We therefore used human liver microsomes (HLMs) and cDNA-expressed human cytochrome P450s (rCYPs) to study in vitro inhibition of buprenorphine metabolism to norbuprenorphine (CYP3A4 and 2C8)

Pharmacologic Profile of Naloxegol, a Peripherally Acting µ-Opioid Receptor Antagonist, for the Treatment of Opioid-Induced Constipation.

Eike Floettmann et al.

The Journal of pharmacology and experimental therapeutics, 361(2), 280-291 (2017-03-25)

Opioid-induced constipation (OIC) is a common side effect of opioid pharmacotherapy for the management of pain because opioid agonists bind to µ-opioid receptors in the enteric nervous system (ENS). Naloxegol, a polyethylene glycol derivative of naloxol, which is a derivative

Small expression tags enhance bacterial expression of the first three transmembrane segments of the apelin receptor.

Aditya Pandey et al.

Biochemistry and cell biology = Biochimie et biologie cellulaire, 92(4), 269-278 (2014-06-20)

G-protein coupled receptors (GPCRs) are inherently dynamic membrane protein modulators of various important cellular signaling cascades. The apelin receptor (AR or APJ) is a class A GPCR involved in numerous physiological processes, implicated in angiogenesis during tumour formation and as

Assessing in vitro and in vivo Trogocytosis By Murine CD4+ T cells.

Jim Reed et al.

Bio-protocol, 10(9), e3607-e3607 (2021-03-05)

Recognition of antigens by lymphocytes (B, T, and NK) on the surface of an antigen-presenting cell (APC) leads to lymphocyte activation and the formation of an immunological synapse between the lymphocyte and the APC. At the immunological synapse APC membrane

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