Biologics and Biosimilars Characterization

应用筛选条件

facet applications:Biologics and Biosimilars Characterization

facet applications:Large molecule HPLC

facet applications:Mass spectrometry

facet applications:Protein mass spectrometry

facet content type:Protocol

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Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

Performing a Separation with Sephadex^®

This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

MS Metabolite Library Protocol

MSMLS™ offers high-purity small biochemical molecules for broad primary metabolism studies in an economical, ready-to-use format.

Cationic Polymers HPLC on TSKgel^® PWXL-CP Columns

Separation of various acids and compounds by molecular weight: PAA, PEI, PIDADMACl, PAS, cationic dextran, chitosan

Determination of L- and D-Lactic Acid Enantiomers by HPLC/MS/MS

Rapid, simple, reproducible chiral separation and quantification of L- and D-lactic acid enantiomers using Supelco’s Astec CHIROBIOTIC® R chiral column and tandem MS.

Workflow and Analysis of Stressed and Unstressed Antibody Samples By Size Exclusion Chromatography

A complete SEC-UV workflow for quantifying monoclonal antibody fragments, monomers, and aggregates, including procedure for a system suitability test and heat stressing of antibodies.

Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody

A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.

Released N-Linked Glycan Analysis Workflow of Adalimumab

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

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