Gene expression and silencing

应用筛选条件

facet applications:Gene expression and silencing

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Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

esiRNA Knockdown Efficiency Tested by Western Blotting

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

默克Sigma-Aldrich® Advanced Genomics提供了一整套用于基因敲减和过表达实验的优化CRISPRi和CRISPRa文库目前可提供CRISPRi和SAM CRISPRa混合的慢病毒文库，也可根据您的特定需求提供定制产品。

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Genome Editing in Plants with CRISPR/Cas9

ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

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