Low pressure liquid chromatography

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facet applications:Low pressure liquid chromatography

facet content type:Technical Article

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Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protein G and Protein A Bind to Different IgG

This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Capto Adhere Anion Exchanger for MAbs & Bind/Elute

Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.

Capto MMC

Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.

Characteristics of Dextrin Sepharose® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.

Hydrophobic Interaction Chromatography Setup

Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode

In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

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