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Primary cell culture

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facet applications:Primary cell culture
facet content type:Protocol
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Primary Human Spheroid-Qualified Plateable Hepatocyte Culture Protocol
Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.
Human Epidermal Melanocytes (HEM) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Culturing Human Aortic Smooth Muscle Cells
Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.
Human Mesenchymal Stem Cells & Growth Media
Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.
Human Chondrocytes (HC) Culture Protocol
Cryovial handling precautions prevent injuries from liquid nitrogen bursts.
Ficoll® 400 for Gradient Centrifugation
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
ADCC Protocol using BioIVT Cryopreserved NK Effector Cells
Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.
Human Bronchial Epithelial Cell line
Technical information for working with human bronchial epithelial cell line including thawing, subculturing and cryopreservation.
Human Dermal Fibroblasts (HDF) Culture Protocol
This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF
Human Follicle Dermal Papilla Cells (HFDPC) Culture Protocol
Technical information for working with human follicle dermal papilla cells including thawing, subculturing and cryopreservation.
Human Astrocytes (HA) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Mononuclear Cells – Periphery Blood (HMNC-PB) Culture Protocol
Technical information for working with human mononuclear cells – periphery blood including thawing, subculturing and cryopreservation
Human Osteoblasts (HOb) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Bovine Aortic Endothelial Cells (BAOEC) Culture Protocol
Technical information for working with bovine aortic endothelial cells including thawing, subculturing and cryopreservation.
Human Pulmonary Artery Smooth Muscle Cells (HPASMC) Culture Protocol
Technical information for working with human pulmonary artery smooth muscle cells including thawing, subculturing and cryopreservation
Human Pulmonary Artery Endothelial Cells (HPAEC) Culture Protocol
Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation
Human Preadipocytes (HPAd) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Fibroblast-Like Synoviocytes (HFLS) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Mammary Epithelial Cells (HMEpC) Culture Protocol
Mammary Epithelial Cells and primary epithelial cells from other tissue at Cell Applications, your worldwide provider of human & animal cells, antibodies.
Human Lung Microvascular Endothelial Cells (HLMVEC) Culture Protocol
Technical information for working with human lung microvascular endothelial cells including thawing, subculturing and cryopreservation
HCF Culture Protocol
Culturing HCFs from human heart ventricles involves critical steps for studying cardiac-related conditions.
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