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Protein concentration and buffer exchange

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facet applications:Protein concentration and buffer exchange
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Concentrating Large Volumes With Centricon® Plus-70 Centrifugal Filter Device
Large-volume centrifugal filter devices are a convenient alternative to stirred cell ultrafiltration devices for purifying proteins from large volumes of solution.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Dialysis Tubing
Dialysis Tubing
Concentration & Buffer Exchange with Amicon® Cells
Amicon Stirred Cells concentrate and buffer-exchange macrosolutes efficiently with pressure-driven filtration.
Protein Concentration for Large Volumes Samples
For filtering large volumes of biological solutions, we manufacture specialized equipment including centrifugal filtering devices, discs, cells and solvents.
Protein Sample Ultrafiltration
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Salt Selection & Buffer Preparation
Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.
Practical Considerations for IEX Separation
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Amicon® Pro Purification System – Protein Purification
Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
Rapid Antibody Concentration with Amicon® Ultra Centrifugal Filters
Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
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