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Protein quantitation
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facet applications:Protein quantitation
facet content type:Protocol
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Protein Determination Modified Lowry Method
To standardize a procedure for the determination of protein by modified Lowry.
Protein Determination by the Warburg-Christian Method
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Protein Determination by the Bicinchoninic Acid (BCA) Method
A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.
Protein Determination using 2-D Quant Kit
2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.
Creating PLA® Probes
Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Duolink® PLA Brightfield Protocol
Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
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