Protein quantitation

SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

How to Create PLA® Probes

Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue.

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Protein Determination using 2-D Quant Kit

2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Duolink® PLA Brightfield Protocol

Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Duolink® PLA Flow Cytometry Protocol

Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.

Protein Determination by the Bicinchoninic Acid (BCA) Method

A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

Production of 15N-13C Dual-labelled Proteins

This protocol is based on an endogenous amino acid suppression method and shows a six-fold increase in 15N, methionine-(methyl-13C) dual-labelled protein when compared with M9 medium.