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Advanced gene editing
应用筛选条件
facet applications:Advanced gene editing
facet content type:Protocol
显示 1-14 共 14 条结果
利用 CRISPR-Cas9 基因组编辑技术创建转基因小鼠
使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。
CRISPR / Cas核酸酶RNA介导的基因组编辑
了解CRISPR Cas9、定义和工作原理。CRISPR是一种全新、实惠的基因组编辑工具，可让所有人掌握基因组编辑。
植物CRISPR/Cas9基因组编辑
锌指核酸酶（ZFN）是由Sigma-Aldrich在不到8年前推出的，但是从那时起靶向基因组编辑技术已迅速发展。最近，CRISPR/Cas9 通路的发现加快了对该领域的兴趣，为研发开辟了新的可能性。
PURedit™ CRISPR合成引导RNA和Cas9蛋白
可靠的PURedit™ CRISPR合成gRNA和Cas9蛋白可提供行业领先的靶位点切割和特异性
慢病毒转导实验方案
如何利用MISSION shRNA慢病毒颗粒进行慢病毒转导，以实现稳定的长期沉默和表型变化的详细操作步骤。
使用X-tremeGENE HP转染试剂进行慢病毒生产
慢病毒是一种用于在研究应用中转染多种细胞类型的强大工具。
Recombinant eSpCas9 Protein for RNP-Based Genome Editing
The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
Universal Transfection Reagent Protocol
Our Universal Transfection Reagent is a unique formulation of a proprietary polymer blend used for transient and stable transfection of nucleic acids into various eukaryotic cell lines and hard-to-transfect primary cells. This is a fast and easy protocol is compatible
Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein
Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity
Lentiviral Production Using X-tremeGENE HP Transfection Reagent
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field
Lentiviral Transduction Protocol
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.