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Biologics and Biosimilars Characterization

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facet applications:Biologics and Biosimilars Characterization
facet applications:Large molecule HPLC
facet applications:Mass spectrometry
facet applications:Protein mass spectrometry
facet content type:Protocol
显示 1-30 共 113 条结果
质谱代谢物文库实验方案
质谱代谢物文库(MSMLS)是一系列广泛存在于初级代谢的高质量小生化分子。其皆为高纯度(> 95%)化合物,并以经济、即用的形式提供。
使用Sephacryl®进行分离
本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离,该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。
聚糖的质谱
该聚糖质谱分析方案,被称之为干液滴法,基于原始的MALDI实验并依然是MS领域中最常用的方法。
MSTFA/MSTFA-d9苯丙胺的衍生化,用于GC/MS检测和鉴定
示例显示了使用SLB-5ms GC色谱柱的苯丙胺的MSTFA/MSTFA-d9衍生化如何在质谱图和色谱图中提供有价值的信息。
阿达木单抗(adalimumab)肽图谱 LC-MS/MS 分析工作流程
基于优化的 LC-MS/MS 工作流程,通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。
Milli-Q® SQ 2 系列纯水系统安装和使用
观看教学视频并下载 Milli-Q® SQ 2 系列实验室纯水系统用户手册。您将快速掌握,并立即投入使用。在线购买系统、配件、纯化柱和服务。
Amicon® Ultra超滤离心管在MALDI质谱分析中纯化血清多肽
生物标志物在药物发现和开发中起关键作用,
使用Sephadex®进行分离
本页介绍如何使用Cytiva的sephacryl色谱柱进行分离,该色谱柱是由环氧氯丙烷交联葡聚糖组成的尺寸排阻色谱介质。
Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using Chiral HPLC and MS Detection
Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC Columns
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution
HPLC Analysis of Immunoglobulin G (IgG) on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution
Measurement of acetic acid vapor in the workplace using passive sampling SPME and on-fiber derivatization with 1-pyrenyldiazomethane: Comparison with active sampling
Measurement of acetic acid vapor in the workplace using passive sampling SPME and on-fiber derivatization with 1-pyrenyldiazomethane: Comparison with active sampling.
GC Analysis of PBDE Flame-Retardant Compounds
GC Analysis of PBDE Flame-Retardant Compounds
Determination of Quinine in Tonic Water and Caffeine in Coffee and Cola Drinks Using TLC-MS
HPTLC–MS analysis of quinine in tonic water and caffeine in coffee and cola drinks. Tonic and cola drinks decarbonized through ultrasonication, and coffee extracted through PTFE filter.
Qualitative Thin Layer Chromatography Analysis Ginkgo
Qualitative Thin Layer Chromatography Analysis of Flavonoids and Quantification of Terpene Lactones in Ginkgo Biloba Extracts and Tablets
Determination of Lactose in Dairy Products Using TLC-MS
Straightforward HPTLC-MS analysis of lactose in dairy products (milk or yoghurt) using only protein crash, centrifugation and dilution as sample preparation.
Reversed-Phase HPLC Analysis of Insulin Variants and Analogs by UV and Mass Spectral Detection
Insulin is an approximately 6000 Da peptide hormone used to regulate the glucose concentration in blood. There are several different forms of insulin that are both natural and recombinant; the recombinant forms have been engineered to improve pharmacokinetic properties. A
LC/MS Analysis of Nucleotides on SeQuant ZIC-cHILIC
HILIC separation is an alternative that permits sensitive MS detection and without the use of ion-pair reagents.
HPLC Analysis of Isocyanates on Titan™ C18
HPLC Analysis of Isocyanates on Titan™ C18
Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3
We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.
Metabolites – A Serious Challenge for LC/MS Separations and Usual Detection Techniques
We offer the tools for the analysis of the metabolites; including certified reference standards, enzymes, substrates, and chromatographic products.
Antibody Drug Conjugate Mimic Enables LC-MS Method Development Without Risk
Here we show how LC and MS methods may be optimized using a non-toxic surrogate of the ADC, an “ADC-mimic”, that behaves very similarly to the Cys-linked ADC Adcetris (Seattle Genetics).
Mass Spectrometry Metabolite Library Protocol
MSMLS™ (Mass Spectrometry Metabolite Library of Standards) is a collection of high quality small biochemical molecules that span a broad range of primary metabolism. These are high purity (>95%) compounds supplied in an economical, ready-to-use format.
radiello® Diffusive Air Sampling Application - Volatile Organic Compounds (VOCs) Thermally Desorbed
Volatile organic compounds are trapped by adsorption and recovered by thermal desorption, analysis is performed by capillary gas chromatography and FID or MS detection.
GC/MS Analysis of Ethanol in Low Alcohol Beer on SUPELCOWAX® 10 after Headspace SPME using a 100 µm PDMS Fiber
GC/MS Analysis of Ethanol in Low Alcohol Beer on SUPELCOWAX® 10 after Headspace SPME using a 100 μm PDMS Fiber
Clinical Application of SPME: Analysis of VOCs in Exhaled Breath as Cancer Biomarkers
This report demonstrates the potential of solid phase microextraction as a clinical research tool for the extraction of VOCs associated with lung cancer from human breath.
Performing a Separation with Sephacryl®
This page shows how to perform a separation with a sephacryl column from Cytiva which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers
We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.
Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber
Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt
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