Protocol for the seeding and cultivation of primary cells received in cryopreserved or proliferating formats. Information on primary cell culture, cell handling and passaging.

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

Trilineage Differentiation of Multipotent Human Mesenchymal Stem Cells (MSCs) into Osteocytes, Adipocytes and Chondrocytes

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and

Primary Cell Culture FAQ

Primary Cell Culture: Frequently Asked Questions (FAQs)

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Lung Fibroblasts (HLF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Epidermal Keratinocytes (HEK) Culture Protocol

Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK

Human Skeletal Muscle Cells (HSkMC) Culture Protocol

Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation

Rat Skeletal Muscle Cells (RSkMC) Culture Protocol

Technical information for working with rat skeletal muscle cells including thawing, subculturing and cryopreservation

Rat Brain Microvascular Endothelial Cells (RBMVEC) Culture Protocol

Technical information for working with rat brain microvascular endothelial cells including thawing, subculturing and cryopreservation

Human Coronary Artery Endothelial Cells (HCAEC) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Chondrocytes (HC) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room

Culturing Human Aortic Smooth Muscle Cells

Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.

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