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M Daben J Libardo et al.
Biochimie, 113, 143-155 (2015-04-22)
The emergence of antibiotic resistant strains of bacteria has resulted in the need to develop more potent antimicrobials that target microorganisms in a novel manner. Antimicrobial Peptides (AMPs) show great potential for drug development because of their broad activity and
Jenna Oberstaller et al.
Nucleic acids research, 42(13), 8271-8284 (2014-06-25)
We provide the first comprehensive analysis of any transcription factor family in Cryptosporidium, a basal-branching apicomplexan that is the second leading cause of infant diarrhea globally. AP2 domain-containing proteins have evolved to be the major regulatory family in the phylum
Thioflavin T as a fluorescence probe for monitoring RNA metabolism at molecular and cellular levels.
Shinya Sugimoto et al.
Nucleic acids research, 43(14), e92-e92 (2015-04-18)
The intrinsically stochastic dynamics of mRNA metabolism have important consequences on gene regulation and non-genetic cell-to-cell variability; however, no generally applicable methods exist for studying such stochastic processes quantitatively. Here, we describe the use of the amyloid-binding probe Thioflavin T
Jinfeng Zhang et al.
PloS one, 10(7), e0133824-e0133824 (2015-07-29)
To meet the demand for the application of high activity and thermostable cellulases in the production of new-generation bioethanol from nongrain-cellulose sources, a hyperthermostable β-1,4-endoglucase Cel12B from Thermotoga maritima was selected for further modification by gene site-directed mutagenesis method in
Craig Skinner et al.
PloS one, 10(7), e0132419-e0132419 (2015-07-21)
Stx2e is a primary virulence factor in STEC strains that cause edema disease in neonatal piglets. Though Stx2a and Stx2e are similar, many antibody-based Stx detection kits are designed to detect Stx2a and do not recognize the Stx2e subtype. Four
Coilín Boland et al.
Cellular and molecular life sciences : CMLS, 71(24), 4895-4910 (2014-07-12)
Membrane proteins are key elements in cell physiology and drug targeting, but getting a high-resolution structure by crystallographic means is still enormously challenging. Novel strategies are in big demand to facilitate the structure determination process that will ultimately hasten the
Mi Huang et al.
Biochimica et biophysica acta, 1853(8), 1808-1817 (2015-05-06)
The differentiation from constantly dividing epithelial cells into secondary fiber cells is a key step during lens development. Failure in this process, which requires cell proliferation inhibition and cell cycle exit, causes cataract formation. HSF4 (Heat Shock Transcription Factor 4)
Philana V van Summeren-Wesenhagen et al.
Microbial cell factories, 14, 79-79 (2015-06-13)
Microbes are extensively engineered to produce compounds of biotechnological or pharmaceutical interest. However, functional integration of synthetic pathways into the respective host cell metabolism and optimization of heterologous gene expression for achieving high product titers is still a challenging task.
Guiquan Guan et al.
Parasitology, 142(11), 1387-1397 (2015-07-15)
Heat shock protein 90 (HSP90) is a key component of the molecular chaperone complex essential for activating many signalling proteins involved in the development and progression of pathogenic cellular transformation. A Hsp90 gene (BQHsp90) was cloned and characterized from Babesia
Kui Zhang et al.
Molecular immunology, 66(2), 409-417 (2015-05-23)
Cathepsins are the main members of the cysteine family and play important roles in immune response in vertebrates. The Cathepsin O of Bombyx mori (BmCathepsin O) was cloned from the hemocytes by the rapid amplification of cDNA ends (RACE). The
A P Carneiro et al.
British journal of cancer, 111(3), 551-558 (2014-06-18)
This study aimed to identify novel biomarkers for thyroid carcinoma diagnosis and prognosis. We have constructed a human single-chain variable fragment (scFv) antibody library that was selected against tumour thyroid cells using the BRASIL method (biopanning and rapid analysis of
Lívia Czimbalek et al.
FEBS letters, 589(20 Pt B), 3085-3089 (2015-09-09)
The effects of toxofilin (an actin binding protein of Toxoplasma gondii) on G-actin was studied with spectroscopy techniques. Fluorescence anisotropy measurements proved that G-actin and toxofilin interact with 2:1 stoichiometry. The affinity of toxofilin to actin was also determined with
Eun Young Jeon et al.
Biomaterials, 67, 11-19 (2015-07-22)
Currently approved surgical tissue glues do not satisfy the requirements for ideal bioadhesives due to limited adhesion in wet conditions and severe cytotoxicity. Herein, we report a new light-activated, mussel protein-based bioadhesive (LAMBA) inspired by mussel adhesion and insect dityrosine
An-Ci Lin et al.
Veterinary journal (London, England : 1997), 205(2), 254-262 (2015-04-18)
Canine mammary tumors (CMTs) are the most common type of tumors in female dogs. Heat shock proteins are highly expressed in many cancers and are involved in tumor progression and chemoresistance in CMTs; however, the biological role of canine heat
Cheng Wang et al.
Antimicrobial agents and chemotherapy, 62(2) (2017-11-22)
The increasing incidence of multidrug-resistant Acinetobacter baumannii (MDRAb) infections worldwide has necessitated the development of novel antibiotics. Human defensin 5 (HD5) is an endogenous peptide with a complex architecture and antibacterial activity against MDRAb In the present study, we attempted
Andrea Rivas-Aravena et al.
Fish & shellfish immunology, 45(1), 157-166 (2015-04-12)
Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the
Tali H Reingewertz et al.
Biochemistry, 54(21), 3337-3347 (2015-05-13)
The leading risk factor for gastric cancer in humans is infection by Helicobacter pylori strains that express and translocate the oncoprotein CagA into host epithelial cells. Once inside host cells, CagA interacts with ASPP2, which specifically stimulates p53-mediated apoptosis and
Mingxu Zhou et al.
Archives of microbiology, 197(6), 797-803 (2015-05-04)
Lipid rafts are cholesterol- and sphingolipid-rich ordered microdomains distributed in the plasma membrane that participates in mammalian signal transduction pathways. To determine the role of lipid rafts in mediating interactions between enteropathogens and intestinal epithelial cells, membrane cholesterol was depleted
Francesco Balestri et al.
Biochemical and biophysical research communications, 456(4), 891-895 (2014-12-22)
Although glucose is one of the most important physio-pathological substrates of aldose reductase, it is not an easy molecule for in vitro investigation into the enzyme. In many cases alternative aldoses have been used for kinetic characterization and inhibition studies.
Leah M Smith et al.
Nature protocols, 16(9), 4382-4418 (2021-08-06)
We recently developed a high-throughput functional genomics approach, named 'SorTn-seq', to identify factors affecting expression of any gene of interest in bacteria. Our approach facilitates high-throughput screening of complex mutant pools, a task previously hindered by a lack of suitable
Feiye Guo et al.
Journal of cellular biochemistry, 116(10), 2177-2187 (2015-03-05)
Phagocytosis is critical to tissue homeostasis, as highlighted by phagocytosis defect of retinal pigment epithelial (RPE) cells with debris accumulation, photoreceptor degeneration and blindness. Phagocytosis ligands are the key to delineating molecular mechanisms and functional roles of phagocytes, but are
Teddy Kamata et al.
Clinical and vaccine immunology : CVI, 21(12), 1605-1612 (2014-09-19)
Infectious hemorrhagic fevers caused by the Marburg and Ebola filoviruses result in human mortality rates of up to 90%, and there are no effective vaccines or therapeutics available for clinical use. The highly infectious and lethal nature of these viruses
Mi Ha Le et al.
PloS one, 9(7), e102245-e102245 (2014-07-19)
Chitin, an integral component of the fungal cell wall, is one of the best-studied microbe-associated molecular patterns. Previous work identified a LysM receptor-like kinase (LysM-RLK1/CERK1) as the primary chitin receptor in Arabidopsis. In order to identify proteins that interact with
Marcos Leite Santoro et al.
Psychiatry research, 219(3), 674-679 (2014-07-22)
The spontaneously hypertensive rat (SHR) strain was shown to be a useful animal model to study several behavioral, pathophysiological and pharmacological aspects of schizophrenia and attention-deficit/hyperactivity disorder. To further understand the genetic underpinnings of this model, our primary goal in
Avisek Mahapa et al.
PloS one, 10(3), e0122168-e0122168 (2015-03-31)
SarA, a Staphylococcus aureus-specific dimeric protein, modulates the expression of numerous proteins including various virulence factors. Interestingly, S. aureus synthesizes multiple SarA paralogs seemingly for optimizing the expression of its virulence factors. To understand the domain structure/flexibility and the folding/unfolding
Youjun Feng et al.
MicrobiologyOpen, 4(4), 644-659 (2015-06-04)
Recently, we determined that BioR, the GntR family of transcription factor, acts as a repressor for biotin metabolism exclusively distributed in certain species of α-proteobacteria, including the zoonotic agent Brucella melitensis and the plant pathogen Agrobacterium tumefaciens. However, the scenario
M B Camarada et al.
Physical chemistry chemical physics : PCCP, 17(29), 19001-19011 (2015-07-02)
Cationic dendrimers, such as PAMAM, are known to be positively charged at neutral pH allowing their unspecific interaction with proteins and other cellular components. Especially, ferritin, which has an important role in iron homeostasis, presents a negative electrostatic potential at
Barbara Shannon et al.
ACS synthetic biology, 9(10), 2617-2624 (2020-09-24)
We study both in silico and in vivo the real-time feedback control of a molecular titration motif that has been earmarked as a fundamental component of antithetic and multicellular feedback control schemes in E. coli. We show that an external feedback
Yosuke Togashi et al.
Oncotarget, 5(10), 2962-2973 (2014-06-17)
Chromosomal band 11q13 seems to be one of the most frequently amplified lesions in human cancer, including esophageal squamous cell cancer (ESCC). The oral cancer overexpressed 1 (ORAOV1) gene has been identified within this region, but its detailed biological function
Zheng Han et al.
Bioconjugate chemistry, 26(5), 830-838 (2015-04-08)
Extradomain-B fibronectin (EDB-FN), one of the oncofetal fibronectin (onfFN) isoforms, is a high-molecular-weight glycoprotein that mediates cell adhesion and migration. The expression of EDB-FN is associated with a number of cancer-related biological processes such as tumorigenesis, angiogenesis, and epithelial-to-mesenchymal transition
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