Carisbamate blockade of T-type voltage-gated calcium channels.

Carisbamate (CRS) is a novel monocarbamate compound that possesses antiseizure and neuroprotective properties. However, the mechanisms underlying these actions remain unclear. Here, we tested both direct and indirect effects of CRS on several cellular systems that regulate intracellular calcium concentration [Ca We used a combination of cellular electrophysiologic techniques, as well as cell viability, Store Overload-Induced Calcium Release (SOICR), and mitochondrial functional assays to determine whether CRS might affect [Ca In CA3 pyramidal neurons, kainic acid induced significant elevations in [Ca Our data indicate that the neuroprotective and antiseizure activity of CRS likely results in part from decreased [Ca