- DNA sequencing with chain-terminating inhibitors.
DNA sequencing with chain-terminating inhibitors.
Proceedings of the National Academy of Sciences of the United States of America (1977-12-01)
F Sanger, S Nicklen, A R Coulson
PMID271968
摘要
A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.