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Merck
CN
  • Solid-phase microextraction (SPME) analysis of six Italian populations of Ephedra nebrodensis Tineo ex Guss. subsp. nebrodensis.

Solid-phase microextraction (SPME) analysis of six Italian populations of Ephedra nebrodensis Tineo ex Guss. subsp. nebrodensis.

Chemistry & biodiversity (2011-01-25)
Filippo Maggi, Marcello Nicoletti, Valentina Petitto, Gianni Sagratini, Fabrizio Papa, Sauro Vittori
摘要

Headspace solid-phase microextraction (HS-SPME) coupled with GC/FID and GC/MS was applied for the first time in the analysis of the volatile fraction of an Ephedra species. Notably, six Italian populations (Marche, Abruzzo, and Sardinia) of Ephedra nebrodensis subsp. nebrodensis, covering almost the entire Italian area, were investigated to examine the chemical variability and to support the taxonomy of the species. A fiber screening with polymethylsiloxane (PDMS), Carboxen(TM) /polymethylsiloxane (CAR/PDMS), and polymethylsiloxane/divinylbenzene (PDMS/DVB) coatings, together with an optimization of the extraction conditions were carried out before analysis of the six populations. A total of 119 volatiles were identified in the headspace of different samples, accounting for 63.35-100.00% of the total volatiles. A great variability was found in the qualitative composition of different samples, since only 18 components were in common among all populations. The headspace composition was dominated by sesquiterpene hydrocarbons (52.30-88.32%), with β-maaliene (traces-7.49%), β-patchoulene (traces-1.29%), β-panasinsene (traces-6.85%), α-isocomene (traces-31.25%), α-trans-bergamotene (traces-6.95%), alloaromadendrene (traces-33.20%), α-acoradiene (traces-9.41%), and γ-muurolene (0.61-16.33%) being the most abundant constituents. Noteworthy is the occurrence in a sample of two major unknown sesquiterpenes, one hydrocarbon (24.49%, RI: 1396) and one oxygenated compound (10.37%, RI: 1591), whose mass spectra were reported for the first time. Multivariate chemometric techniques, such as cluster analysis (CA) and principal component analysis (PCA), were used to characterize the samples according to the geographical origin.

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Supelco
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder
Supelco
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 24 ga, StableFlex
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SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler
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SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm
Supelco
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler
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ORBO Carboxen-564 管, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)
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Carbopack®吸附剂, matrix Carboxen® 1000, 60-80 mesh, bottle of 10 g
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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga
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ORBO-78 400/200mg,每包 25 支, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea
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Carboxen®1010 多孔层壁涂开管柱, L × I.D. 30 m × 0.53 mm, average thickness 30 μm
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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler
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Carboxen® 1006 多孔层壁涂开管柱, L × I.D. 30 m × 0.53 mm, average thickness 30 μm
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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler
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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler
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Carboxen®1010 多孔层壁涂开管柱, L × I.D. 30 m × 0.32 mm, average thickness 15 μm
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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber
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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler
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SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder
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Carbopack®吸附剂, matrix Carboxen® 572, 20-45 mesh, bottle of 10 g
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Carboxen® 1006 多孔层壁涂开管柱, L × I.D. 30 m × 0.32 mm, average thickness 15 μm
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Tenax® TA/Carboxen® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok® End-Fittings), pkg of 10 ea
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Carboxen® 1000 双向固相萃取管, 200 mg/mL, pk 30
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Carbopack®吸附剂, matrix Carboxen® 1000, 40-60 mesh, bottle of 50 g
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Carbopack®吸附剂, matrix Carboxen® 1003, 40-60 mesh, bottle of 10 g
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