- Extraction and Activity of O-acetylserine(thiol)lyase (OASTL) from Microalga Chlorella sorokiniana.
Extraction and Activity of O-acetylserine(thiol)lyase (OASTL) from Microalga Chlorella sorokiniana.
Bio-protocol (2017-06-20)
Giovanna Salbitani, Simona Carfagna
PMID34541094
摘要
O-acetylserine(thiol)lyase (OASTL) is an enzyme catalysing the reaction of inorganic sulphide with O-acetylserine to form the S-containing amino acid L-cysteine. Here we describe an improved protocol to evaluate the activity of this enzyme from the microalga Chlorella sorokiniana. It is a colorimetric assay based on the reaction between cysteine, the product of OASTL activity, and ninhydrin reagent, which forms a thiazolidine (Thz).
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磷酸钾 一元, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
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氯化钠, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
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硼酸, BioReagent, Molecular Biology, suitable for cell culture, suitable for plant cell culture, ≥99.5%
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氯化钙, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%