跳转至内容
Merck
CN
  • Unbiased cell quantification reveals a continued increase in the number of neocortical neurones during early post-natal development in mice.

Unbiased cell quantification reveals a continued increase in the number of neocortical neurones during early post-natal development in mice.

The European journal of neuroscience (2007-09-28)
Lise Lyck, Thomas Krøigård, Bente Finsen
摘要

The post-natal growth spurt of the mammalian neocortex has been attributed to maturation of dendritic arborizations, growth and myelination of axons, and addition of glia. It is unclear whether this growth may also involve recruitment of additional neurones. Using stereological methods, we analysed the number of neurones and glia in the neocortex during post-natal development in two separate strains of mice. Cell counting by the optical fractionator revealed that the number of neurones increased 80-100% from the time of birth to post-natal day (P)16, followed by a reduction by approximately 25% in the young adult mouse at P50-55. Unexpectedly, at the time of birth less than half of the neurones and at P8 only 65% of the neurones expressed neuronal nuclear antigen (NeuN), a marker of mature post-migratory neurones. In accordance with these observations, NeuN acquisition by neurones in layer VIa was delayed until P16. The number of glia reached its maximum at P16, whereas the number of oligodendroglia, identified using a transgenic marker, increased until P55, the latest time of observation. Neurones continued to accumulate in the developing neocortex during the first 2 weeks of post-natal development, underscoring fundamental differences in brain development in the mouse compared with human and non-human primates. Further, delayed acquisition of NeuN by neurones in the deepest neocortical layers and continued addition of oligodendroglia to the neocortex suggested that neocortical maturation should be regarded as an ongoing process continuing into the young adult mouse.

材料
产品编号
品牌
产品描述

Sigma-Aldrich
抗NG2硫酸软骨素蛋白聚糖抗体, Chemicon®, from rabbit
Sigma-Aldrich
抗微管蛋白抗体,βIII亚型,计算机X线体层,克隆TU-20(类似于TUJ1), ascites fluid, clone TU-20 (Similar to TUJ1), Chemicon®
Sigma-Aldrich
抗-NeuN抗体,克隆A60,生物素结合, clone A60, Chemicon®, from mouse