- High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes.
High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes.
Methods in molecular biology (Clifton, N.J.) (2021-09-22)
Lilian Vincis Pereira Sanglard, Catherine Colas des Francs-Small
PMID34545490
摘要
Blue native electrophoresis (BN-PAGE) is a highly resolutive method suited to the study of high molecular weight protein complexes between 100 and >3000 kDa. One of the drawbacks of this method is that it is very time-consuming and requires high quantities of purified organelles. Here we describe a high throughput BN-PAGE method allowing to screen libraries of plants potentially altered in respiratory metabolism.