- Molecular and cellular approaches for establishment of an in vitro spermatogenesis system.
Molecular and cellular approaches for establishment of an in vitro spermatogenesis system.
Methods in molecular biology (Clifton, N.J.) (2004-03-24)
Karim Nayernia, Wolfgang Engel
PMID15037792
材料
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Sigma-Aldrich
胰蛋白酶-EDTA 溶液, 0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
Sigma-Aldrich
胶原酶 来源于溶组织梭菌, 0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid