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Merck
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A Modified Handmade Cloning Method for Dromedary Camels.

Methods in molecular biology (Clifton, N.J.) (2023-04-12)
Fariba Moulavi, Sayyed Morteza Hosseini
摘要

Camels play very important economic and sociocultural roles for communities residing in arid and semi-arid countries. The positive impacts of cloning on genetic gain in camel species are indisputable, considering the unique ability of cloning to produce a large number of offspring of a predefined sex and genotype using somatic cells obtained from elite animals, live or dead, and within any age category. However, the current low efficiency of camel cloning seriously limits its commercial applicability. We have systematically optimized technical and biological factors for dromedary camel cloning. In this chapter, we present the details of our current standard operating procedure for dromedary camel cloning, namely, "modified handmade cloning (mHMC)."

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Trichostatin A, Ready Made Solution, 5 mM in DMSO, from Streptomyces sp.