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Merck
CN
  • Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivatives.

Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivatives.

Regenerative therapy (2023-10-20)
Yuki Ikeda, Shoichiro Tani, Takeshi Moriishi, Aiko Kuroda, Yuki Matsuo, Naoya Saeki, Chizuko Inui-Yamamoto, Makoto Abe, Takashi Maeda, David W Rowe, Ung-Il Chung, Hironori Hojo, Yuki Matsushita, Takashi Sawase, Shinsuke Ohba
摘要

Vertebrates form their skeletal tissues from three distinct origins (the neural crest, paraxial mesoderm, and lateral plate mesoderm) through two distinct modes of ossification (intramembranous and endochondral ossification). Since the paraxial mesoderm generates both intramembranous and endochondral bones, it is thought to give rise to both osteoprogenitors and osteo-chondroprogenitors. However, it remains unclear what directs the paraxial mesoderm-derived cells toward these different fates in distinct skeletal elements during human skeletal development. To answer this question, we need experimental systems that recapitulate paraxial mesoderm-mediated intramembranous and endochondral ossification processes. In this study, we aimed to develop a human pluripotent stem cell (hPSC)-based system that models the human intramembranous ossification process. We found that spheroid culture of the hPSC-derived paraxial mesoderm derivatives generates osteoprogenitors or osteo-chondroprogenitors depending on stimuli. The former induced intramembranous ossification, and the latter endochondral ossification, in mouse renal capsules. Transcriptional profiling supported the notion that bone signatures were enriched in the intramembranous bone-like tissues. Thus, we developed a system that recapitulates intramembranous ossification, and that enables the induction of two distinct modes of ossification by controlling the cell fate of the hPSC-derived paraxial mesoderm derivatives.

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Roche
抗地高辛-AP,Fab片段, from sheep
Sigma-Aldrich
LDN193189 盐酸盐, ≥98% (HPLC)
Sigma-Aldrich
平滑激动剂,SAG, A cell-permeable Smoothened Agonist, SAG, CAS 364590-63-6, modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM).