跳转至内容

CN

[Effect of univalent cations on synthesis of surfactants by Acinetobacter calcoaceticus IMV B-7241].

[Effect of univalent cations on synthesis of surfactants by Acinetobacter calcoaceticus IMV B-7241].

Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) (2013-06-01)

T P Pirog, T A Shevchuk, S I Antoniuk, E Iu Kravchenko, G A Iutinskaia

摘要

The effect of univalent cations on activity of key enzymes of C2-metabolism has been investigated in the producer of biosurfactants, Acinetibacter calcoaceticus IMV B-7241 grown on ethanol. It was established that potassium cations are inhibitors of pyroquinolinequinone-dependent alcohol- and acetaldehyde dehydrogenases, the enzymes of biosynthesis of surface-active aminolipids (NADP-dependent glutamate dehydrogenase) and glycolipids (phosphoenopyruvate (PhEP)-carboxikinase), while ammonium cations are activators of these enzymes and PhEP-carboxylase. A decrease of potassium cations concentration in the cultivation medium to 1 mM and increase of the content of amine nitrogen to 10 mM as a result of potassium nitrate substitution by equimolar, as to nitrogen, urea concentration were accompanied by the increase of activity of enzymes of ethanol metabolism and SAS biosynthesis, as well as by the 2-fold increase of conditional concentration of the biosurfactants.

材料

产品编号

品牌

产品描述

Sigma-Aldrich

乙醇，Pure 200纯度, Molecular Biology

Sigma-Aldrich

纯乙醇, 200 proof, ACS reagent, ≥99.5%

Sigma-Aldrich

纯乙醇, 200 proof

Sigma-Aldrich

纯乙醇, 200 proof, meets USP testing specifications

Sigma-Aldrich

纯乙醇, 200 proof, anhydrous, ≥99.5%

Sigma-Aldrich

乙醇，Pure 190纯度, for molecular biology

Sigma-Aldrich

尿素, powder, BioReagent, Molecular Biology, suitable for cell culture

Sigma-Aldrich

纯乙醇, 190 proof, ACS spectrophotometric grade, 95.0%

Sigma-Aldrich

尿素, ACS reagent, 99.0-100.5%

Sigma-Aldrich

酒精, BioUltra, Molecular Biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)

Sigma-Aldrich

酒精试剂, denatured, suitable for HPLC

Supelco

尿素, 8 M (after reconstitution with 16 mL high purity water)

Sigma-Aldrich

尿素溶液, BioUltra, ~8 M in H₂O

Sigma-Aldrich

酒精试剂, reagent grade

Sigma-Aldrich

尿素, ReagentPlus^®, ≥99.5%, pellets

Supelco

无水乙醇, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

尿素, BioUltra, Molecular Biology, 99% (T)

Sigma-Aldrich

纯乙醇, 190 proof, meets USP testing specifications

Sigma-Aldrich

乙醇，变性, reagent grade, denatured

Sigma-Aldrich

尿素, BioXtra, pH 7.5-9.5 (20 °C, 5 M in H₂O)

Sigma-Aldrich

酒精, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL¹, ~96% (based on denaturant-free substance)

Supelco

酒精, standard for GC

Sigma-Aldrich

尿素, suitable for electrophoresis

Supelco

乙醇-50标准液 CRM, 50 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

Supelco

乙醇-300标准液 CRM, 300 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

Sigma-Aldrich

酒精试剂, anhydrous, ≤0.003% water

Sigma-Aldrich

尿素, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)

Supelco

乙醇-10标准液 CRM, 10 mg/dL in H₂O, pack of 10 × 1.2 mL ampules, certified reference material, Cerilliant^®

Supelco

乙醇-80 标准液 CRM, 80 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

Supelco

乙醇-100（10支/盒)标准液 CRM, 100 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®