Merck
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  • Liquid chromatographic analysis of propranolol enantiomers in human blood using precolumn derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate.

Liquid chromatographic analysis of propranolol enantiomers in human blood using precolumn derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate.

Journal of chromatography (1991-10-04)
A Roux, G Blanchot, A Baglin, B Flouvat
摘要

A method for the determination of the R-(+) and S-(-) enantiomers of propranolol in blood was developed. After extraction with heptane-isopentanol and derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate, excess reagent was removed using solid-phase extraction. The enantiomers were separated on an achiral, reversed-phase, radially compressed column, and detected by fluorescence with excitation and emission wavelengths of 260 and 340 nm, respectively. The limit of quantification was 0.5 ng/ml. This method was used for pharmacokinetic analysis of propranolol enantiomers after administration of immediate-release (80 mg) or sustained-release (160 mg) racemic propranolol.

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Sigma-Aldrich
氯甲酸(+)-1-(9-芴)乙酯 溶液, 18 mM in acetone, for chiral derivatization
Supelco
氯甲酸(+)-1-(9-芴)乙酯 溶液, ≥18 mM in acetone, for chiral derivatization, LiChropur