Biodegradation of 2-chloroaniline, 3-chloroaniline, and 4-chloroaniline by a novel strain Delftia tsuruhatensis H1.

A new strain Delftia tsuruhatensis H1 able to degrade several chloroanilines (CAs) as individual compounds or a mixture was isolated from a CA-degrading mixed bacterial culture. The isolated strain could completely degrade 3-CA and 4-CA as growth substrates, while concurrently metabolize 2-CA by growing on other CA compounds. The strain could also efficiently degrade all the three CA components when presented as a mixture. Following CA consumption, stoichiometric amounts of chloride were released and small amount of soluble metabolites accumulated in the medium, indicating that the loss of CA was mainly via mineralization and incorporation into cell material. The additions of yeast extract, citrate or succinate appeared to accelerate CA degradation. In contrast, aniline strongly inhibited the CA degradation. The strain H1 could also decompose other substituted aniline compounds such as 3,4-dichloroaniline, 4-methylaniline, 2,3-dichloroaniline and 2,4-dichloroaniline. The elimination of these CA compounds seemed to occur via an ortho-cleavage pathway.