跳转至内容
Merck
CN
  • Kinetics and thermodynamics of 1-anilino-8-naphthalene sulfonate interactions with Urinary Trypsin Inhibitor.

Kinetics and thermodynamics of 1-anilino-8-naphthalene sulfonate interactions with Urinary Trypsin Inhibitor.

The protein journal (2012-08-10)
Zhenyu Zuo, Handong Fan, Jianjun Guo, Wei Zhou, Lingling Li
摘要

The interactions between Urinary Trypsin Inhibitor (UTI) and 1-anilino-8-naphthalene sulfonate (ANS) were investigated by fluorescence spectra, isothermal titration calorimetry and molecular modeling. The results revealed the presence of four specific binding sites for ANS on UTI, with interactions driven mainly by electrostatic forces. The four specific binding sites indicated the involvement of four hydrophobic patches on UTI. Experimental data also confirmed the presence of a further five nonspecific binding sites that interacted mainly by the formation of salt bridges between the sulfonates of ANS and positive residues on the surface of UTI.

材料
货号
品牌
产品描述

Sigma-Aldrich
8-苯胺基-1-萘磺酸 铵盐, for fluorescence, ≥97.0% (HPLC)
Sigma-Aldrich
8-苯胺基-1-萘磺酸
Sigma-Aldrich
8-苯胺基-1-萘磺酸 半镁盐 水合物, for fluorescence, ≥95.0% (T)
Sigma-Aldrich
8-苯胺基-1-萘磺酸 铵盐, technical, ≥90% (NT)
Sigma-Aldrich
8-苯胺基-1-萘磺酸 半镁盐 水合物, for fluorescence, ≥95% (TLC)