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Merck
CN
  • Discrimination of isobaric Leu/Ile residues by MALDI in-source decay mass spectrometry.

Discrimination of isobaric Leu/Ile residues by MALDI in-source decay mass spectrometry.

Journal of the American Society for Mass Spectrometry (2013-01-12)
Daiki Asakawa, Nicolas Smargiasso, Edwin De Pauw
摘要

MALDI in-source decay (ISD) has been used for top-down sequencing of proteins. The use of the matrix 1,5-diaminonapthalene (1,5-DAN) gave abundant w ions, which are formed from the unimolecular dissociation of z• radical fragments via α cleavage reaction and thus help identify which of the isobaric amino acids, Leu or Ile, is present. The high abundance of w ions in MALDI-ISD with 1,5-DAN results from the low collision rate in the MALDI plume. MALDI-ISD with 1,5-DAN appears to be an useful method for the top-down sequencing of proteins, including discrimination of Leu and Ile near the C-terminal end.

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L -异亮氨酸, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
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2,5-二羟基苯甲酸, suitable for matrix substance for MALDI-MS, >99.0% (HPLC)
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L -异亮氨酸
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L -异亮氨酸, BioUltra, ≥99.5% (NT)
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L -异亮氨酸, 99%, FCC, FG
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1,5-二氨基萘, suitable for matrix substance for MALDI-MS, ≥99.0% (HPLC)
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2,5-二羟基苯甲酸, suitable for matrix substance for MALDI-MS, ≥99.5% (HPLC), Ultra pure
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L -异亮氨酸, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland