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DNA isolation from mammalian samples.

DNA isolation from mammalian samples.

Current protocols in molecular biology (2013-04-03)

Ximeng Liu, Shuko Harada

摘要

DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE) tissue samples.

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氯仿, contains 100-200 ppm amylenes as stabilizer, ≥99.5%

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