- Synthesis and characterization of phosphodiester stationary bonded phases for liquid chromatography.
Synthesis and characterization of phosphodiester stationary bonded phases for liquid chromatography.
Talanta (2015-06-17)
Szymon Bocian, Bogusław Buszewski
PMID26078125
摘要
The new type of stationary bonded phases for liquid chromatography with immobilized artificial membrane properties was synthesized. Based on the modification of diol-bonded silica gel the phosphodiester stationary phases were obtained. The structures of synthesized material were confirmed by different physico-chemical techniques such as elemental analysis, infrared spectroscopy (FTIR), (13)C CP/MAS NMR and in reversed phase and hydrophilic interaction liquid chromatography, where hydrophobic and polar compounds were sufficiently separated. To present the influence of phosphate group on the retention properties, obtained material were compared with Diol-Ester C12 stationary phase.
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Sigma-Aldrich
苯酚 溶液, BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, Molecular Biology
Sigma-Aldrich
苯酚, puriss., meets analytical specification of Ph. Eur., BP, USP, ≥99.5% (GC), crystalline (detached)
Sigma-Aldrich
苯酚 溶液, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology
Sigma-Aldrich
苯酚, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
Sigma-Aldrich
甲醇 溶液, suitable for NMR (reference standard), 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.