- Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission.
Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission.
Nature communications (2017-06-20)
Johannes Schöneberg, Martin Lehmann, Alexander Ullrich, York Posor, Wen-Ting Lo, Gregor Lichtner, Jan Schmoranzer, Volker Haucke, Frank Noé
PMID28627515
摘要
Clathrin-mediated endocytosis (CME) involves membrane-associated scaffolds of the bin-amphiphysin-rvs (BAR) domain protein family as well as the GTPase dynamin, and is accompanied and perhaps triggered by changes in local lipid composition. How protein recruitment, scaffold assembly and membrane deformation is spatiotemporally controlled and coupled to fission is poorly understood. We show by computational modelling and super-resolution imaging that phosphatidylinositol 3,4-bisphosphate [PI(3,4)P