- Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis.
Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis.
Electrophoresis (2017-08-05)
Ederson Paulo Xavier Guilherme, Jocilane Pereira de Oliveira, Lorendane Millena de Carvalho, Igor Viana Brandi, Sérgio Henrique Sousa Santos, Gleidson Giordano Pinto de Carvalho, Junio Cota, Bruna Mara Aparecida de Carvalho
PMID28777449
摘要
A bioreactor was built by means of immobilizing alpha-amylase from Aspergillus oryzae by encapsulation, through cryopolymerization of acrylamide monomers for the continuous starch hydrolysis. The starch hydrolysis was evaluated regarding pH, the concentration of immobilized amylase on cryogel, the concentration of starch solution and temperature. The maximum value for starch hydrolysis was achieved at pH 5.0, concentration of immobilized enzyme 111.44 mg