GC Analysis of Glycerides in Butter on SLB®-35ms
CONDITIONS
column
SLB-35ms, 10 m x 0.25 mm I.D., 0.10 μm; made by cutting down a 30 m x 0.25 mm I.D., 0.10 μm column (29802-U)
oven
75 °C (0.1 min), 15 °C/min to 380 °C (10 min)
inj. temp.
track oven: 75 °C (0.1 min), 15 °C/min to 380 °C (10 min)
detector
FID, 380 °C
carrier gas
helium, 1.5 mL/min
injection
1μL, on-column
sample
butter, melted then diluted, 0.1% (v/v) in chloroform
说明
分析说明
Triglycerides are the main constituent of vegetable oil and animal fat, and make up most of the fats digested by humans. Most natural fats contain a complex mixture of individual triglycerides. Therfore, efficient capillary GC columns are required for ample resolution and subsequent identification. Triglycerides are large compounds, and require a relatively high final oven temperature for GC analysis. For the analysis of triglycerides in butter, a short, thin film version of SLB-35ms (cut-down from a longer column) run to a final oven temperature of 380 ºC was found to provide good resolution and reasonable elution times.
法律信息
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany