Solid Phase Microextraction
5 g sample in 10 mL vial
SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS) (57319)
5 min, headspace, 40 °C
3 min, 320 °C; split 10:1
fiber post bake: 2 min, 320 °C
Supel-Q™ PLOT, 30 m x 0.32 mm I.D. (24242)
50 °C (5 min), 10 °C/min to 230 °C (5 min)
320 °C
MSD
260 °C
m/z 29-200
helium, 2 mL/min constant flow
split, 10:1
SPME, 0.75 mm I.D.
Marijuana oil is produced by extraction of the flower buds of the cannabis sativa plant. This process often involves the use of solvents such as hexane or some alcohols. Even though measures are taken to remove these solvents after extraction, some residual material may remain behind in the final product. In this work, headspace SPME was used to analyze residual solvents from an extract of hemp. For the analysis, hemp extract was used for testing in lieu of marijuana extract, which could not be obtained in the location where the work was done. Hemp and marijuana are both varieties of cannabis sativa, however hemp is a variety that is very low in THC content.
Carboxen is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel-Q is a trademark of Sigma-Aldrich Co. LLC