GC Analysis of cis/trans C18:1, C18:2, and C18:3 FAME Isomers on SP^®-2560 (75 m x 0.18 mm I.D., 0.14 μm), Fast GC Analysis

column

SP-2560, 75 m x 0.18 mm I.D., 0.14 μm (23348-U)

oven

180 °C, isothermal

inj. temp.

220 °C

detector

FID, 220 °C

carrier gas

hydrogen, 25 cm/sec @ 180 °C

injection

0.5 μL, split 100:1

liner

4 mm I.D. split, cup design

sample

mixture of C18:1, C18:2, and C18:3 FAMEs in methylene chloride