HPLC Analysis of Chlorthalidone Enantiomers on Astec^® CYCLOBOND I 2000 HP-RSP

column

CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)

mobile phase

[A] 10 mM ammonium acetate, pH 4.0; [B] acetonitrile; (85:15, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 235 nm

injection

2 μL

sample

5 mg/mL in methanol