[A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
0 to 60% in 20 min
0.3 mL/min
25 °C
UV, 280 nm
5 μL
ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
Proteomix WCX-NP5, 5 cm x 4.6 mm I.D., 5 μm particles (Z777193)
420 psi total pressure (29 bar)
Four commonly studied proteins were chromatographed using a Proteomics WCX-NP5 column to examine retention time and resolution. It was found that the Proteomics WCX-NP5 column does not resolve the proteins as well as the Proteomics SCX-NP5, strong cation exchange column.
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