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LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express OH5

LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express OH5 application for LC-MS

材料

分析色谱柱

产品编号
说明
价格

53757-U

Ascentis® Express OH5, 2.7 μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

标准

产品编号
说明
价格

E-015

乙基-β-D-葡萄糖醛酸标准液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

E-064

乙基硫酸钠标准液 钠盐

1.0 mg/mL in methanol (as ethyl sulfate), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)

mobile phase

5 mM ammonium formate in 95:5, acetonitrile:water, adjusted to pH 4.0 with formic acid

flow rate

0.4 mL/min

pressure

1200 psi (83 bar)

column temp.

35 °C

detector

MS, ESI(-), combined XIC, 125.0 m/z, 221.1 m/z

injection

10 μL

sample

ethyl sulfate (2 μg/mL), ethyl-β-D-glucuronide (20 μg/mL) in 98:2, acetonitrile:methanol

说明

分析说明

The compounds ethyl sulfate and ethyl-β-D-glucuronide are metabolites of ethanol. Their analysis is of clinical interest as biomarkers or indicators of alcohol-induced liver disease (ALD). The HPLC analysis of these compounds using reversed-phase mode on C18 columns suffers from poor retention due to the compounds′ polar nature. However, by using HILIC mode on an Ascentis Express OH5 column, both retention and MS-compatibility are improved. Cerilliant CRMs provided reliable quantification.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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