Performance Comparison of Estapor® Red Intense Microspheres vs. Colloidal Gold in Lateral Flow Immunoassays
Lateral flow immunoassays (LFIA) are essential diagnostic tools celebrated for their simplicity and rapid results, significantly advancing disease detection in point-of-care settings. However, challenges such as achieving higher sensitivity and ensuring reliable outcomes remain. This article highlights the advantages of Estapor® Red Intense microspheres over colloidal gold, showcasing how they elevate LFIA performance.
Importance of Accurate Diagnosis and the Role of Lateral Flow Immunoassays in Public Health
Accurate and early diagnosis is vital for effective public health management, especially underscored during the COVID-19 pandemic. Sensitivity is a critical measure of a diagnostic test's ability to accurately identify individuals with the target analyte. A highly sensitive test minimizes false negatives and enhances patient outcomes. This report illustrates how Estapor® Red Intense microspheres can improve the performance and reliability of lateral flow immunoassays.
Key Advantages of Estapor® Red Intense Microspheres
- Increased Sensitivity: Estapor® Red Intense microspheres detect lower analyte concentrations, achieving test line intensities up to four times stronger than those provided by colloidal gold.
- Faster Results: These microspheres enable visualization of results up to five times quicker, particularly at low analyte concentrations, enhancing diagnostic efficiency.
- Reliable Performance: The precision of results using Estapor® Red Intense microspheres is comparable to colloidal gold, ensuring reliability and reproducibility in various assay conditions.
Performance Comparison of Estapor® Red Intense Microspheres vs. Colloidal Gold in LFIA Applications
In our comparative study, we evaluated the performance of Estapor® Red Intense microspheres against colloidal gold in a FluA antigen LFIA, focusing on key parameters such as sensitivity, precision, and speed of detection.
Enhanced Sensitivity of Estapor® Red Intense Microspheres
The sensitivity of both detector particles was assessed by adding increasing concentrations of the target analyte. Results indicated that the limit of visual detection using Estapor® Red Intense microspheres was as low as 6.6×103 TCID50/mL, while colloidal gold was not visible at this concentration. Estapor® microspheres consistently provided higher test line intensity values across all tested concentrations.
Figure 1: FluA Analyte Detection with Estapor® Red Intense vs. Colloidal Gold
Figure 2: Comparison of Test Line Intensity for Estapor® Red Intense vs. Colloidal Gold
Rapid Speed of Detection with Estapor® Red Intense Microspheres
The speed of test line appearance is crucial in LFIA performance. In our study, Estapor® Red Intense microspheres allowed for faster reading of test results, particularly at low analyte concentrations, where results were obtained more than five times quicker than with colloidal gold.
Figure 3: Test Line Appearance Speed of Estapor® Red Intense vs. Colloidal Gold
Cost-Effectiveness of Estapor® Red Intense Microspheres vs. Colloidal Gold
Cost is an important consideration when selecting detector particles. Estapor® Red Intense microspheres emerged as a more economical option, offering a 24% reduction in conjugated detector particle costs compared to colloidal gold.
Conclusion: Advantages of Estapor® Red Intense Microspheres for Enhanced LFIA Performance
This report compares the performance of Estapor® K1-030 Red Intense microspheres with colloidal gold as detector particles in a model FluA LFIA. The findings support the conclusion that Estapor® K1-030 Red Intense microspheres enhance LFIA performance and reliability, providing superior sensitivity, faster results, and comparable precision.
For detailed experimental procedures and insights, refer to the full protocol available in the Performance Comparison of Estapor® Red Intense Microspheres versus Colloidal Gold as Detector Particles in a FluA Lateral Flow Immunoassay Application Note.
Materials and reagents
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