预设计siRNA

MISSION^®预设计siRNA系与默克公司独家合作，采用独有的Rosetta Inpharmatics siRNA设计算法创建。Rosetta siRNA设计算法利用位点特异性打分矩阵和种子区域知识，预测目标基因最具效率和特异性的序列。该算法的规则基于超过三年的基因沉默实验实证数据开发。

产品优势

同类产品之中的佼佼者，基因沉默效果有保障
有效敲低低丰度信使
使用 11 阳性对照 siRNA 简化转染优化
使用 8 阴性对照 siRNA 区分序列特异性沉默和非特异性效应
数百种经过功能验证的预设计siRNA

产品特性

物种：人类、小鼠和大鼠
规格：2 (10 nmol)、5 (25 nmol)和10 (50 nmol) OD
纯化方法：脱盐或HPLC
序列形式：具有 dTdT 突出端的 21mer 双链体
质检方法：100%质谱分析*
形式：以管装干粉形式提供

*部分生产基地可能采用PAGE方法评估siRNA双链体。

产品库

一种流行的混合形式是 4 种 5 nmol 双链，混合到一支管中（20 nmol 混合）；另一种形式也是 4 种 5 nmol 双链，但每种单管装（20 nmol 单装）。即便如此，我们先进的液体处理器还允许更多组合。如需针对您特定的需求进行可行性评定，请电邮sirnarequest@sial.com。

经验证的siRNA

许多常见基因靶标已经过 ≥75% mRNA 敲低验证（图 1提供了数据示例以及常订购的经验证siRNA清单表（按基因符号）。经验证的siRNA适于转染优化或用作阳性对照。

图 1.用30 nM 浓度的预设计siRNA 转染 HeLa 细胞。转染后 48 小时，通过 qPCR 测定剩余基因表达水平的百分比（相对于模拟物）。数据为四份生物学重复的均值。

材料

精选引用文献

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Salma J, McDermott JC. 2012. Suppression of a MEF2-KLF6 Survival Pathway by PKA Signaling Promotes Apoptosis in Embryonic Hippocampal Neurons. Journal of Neuroscience. 32(8):2790-2803. https://doi.org/10.1523/jneurosci.3609-11.2012

Gilot D, Le Meur N, Giudicelli F, Le Vée M, Lagadic-Gossmann D, Théret N, Fardel O. RNAi-Based Screening Identifies Kinases Interfering with Dioxin-Mediated Up-Regulation of CYP1A1 Activity. PLoS ONE. 6(3):e18261. https://doi.org/10.1371/journal.pone.0018261

Raab M, Kappel S, Krämer A, Sanhaji M, Matthess Y, Kurunci-Csacsko E, Calzada-Wack J, Rathkolb B, Rozman J, Adler T, et al. 2011. Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells. Nat Commun. 2(1): https://doi.org/10.1038/ncomms1395

Chia KM, Liu J, Francis GD, Naderi A. 2011. A Feedback Loop between Androgen Receptor and ERK Signaling in Estrogen Receptor-Negative Breast Cancer. Neoplasia. 13(2):154-166. https://doi.org/10.1593/neo.101324

Ramachandran V, Arumugam T, Langley R, Hwang RF, Vivas-Mejia P, Sood AK, Lopez-Berestein G, Logsdon CD. The ADMR Receptor Mediates the Effects of Adrenomedullin on Pancreatic Cancer Cells and on Cells of the Tumor Microenvironment. PLoS ONE. 4(10):e7502. https://doi.org/10.1371/journal.pone.0007502

Santra MK, Wajapeyee N, Green MR. 2009. F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA damage. Nature. 459(7247):722-725. https://doi.org/10.1038/nature08011

Meng W, Mushika Y, Ichii T, Takeichi M. 2008. Anchorage of Microtubule Minus Ends to Adherens Junctions Regulates Epithelial Cell-Cell Contacts. Cell. 135(5):948-959. https://doi.org/10.1016/j.cell.2008.09.040

Matsubara T, Kida K, Yamaguchi A, Hata K, Ichida F, Meguro H, Aburatani H, Nishimura R, Yoneda T. 2008. BMP2 Regulates Osterix through Msx2 and Runx2 during Osteoblast Differentiation. J. Biol. Chem.. 283(43):29119-29125. https://doi.org/10.1074/jbc.m801774200

10.

Zhou H, Xu M, Huang Q, Gates AT, Zhang XD, Castle JC, Stec E, Ferrer M, Strulovici B, Hazuda DJ, et al. 2008. Genome-Scale RNAi Screen for Host Factors Required for HIV Replication. Cell Host & Microbe. 4(5):495-504. https://doi.org/10.1016/j.chom.2008.10.004

11.

Espeseth AS, Huang Q, Gates A, Xu M, Yu Y, Simon AJ, Shi X, Zhang X, Hodor P, Stone DJ, et al. 2006. A genome wide analysis of ubiquitin ligases in APP processing identifies a novel regulator of BACE1 mRNA levels. Molecular and Cellular Neuroscience. 33(3):227-235. https://doi.org/10.1016/j.mcn.2006.07.003

12.

Bartz SR, Zhang Z, Burchard J, Imakura M, Martin M, Palmieri A, Needham R, Guo J, Gordon M, Chung N, et al. 2006. Small Interfering RNA Screens Reveal Enhanced Cisplatin Cytotoxicity in Tumor Cells Having both BRCA Network and TP53 Disruptions. MCB. 26(24):9377-9386. https://doi.org/10.1128/mcb.01229-06

13.

Majercak J, Ray WJ, Espeseth A, Simon A, Shi X, Wolffe C, Getty K, Marine S, Stec E, Ferrer M, et al. 2006. LRRTM3 promotes processing of amyloid-precursor protein by BACE1 and is a positional candidate gene for late-onset Alzheimer's disease. Proceedings of the National Academy of Sciences. 103(47):17967-17972. https://doi.org/10.1073/pnas.0605461103

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MISSION是德国默克(Merck KGaA, Darmstadt, Germany)及/或其附属公司的商标。标签许可证。

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