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Merck
CN

1.00439

ChromoCult® Listeria Agar Enrichment-Supplement

ChromoCult®, selective for Listeria spp.

别名:

Listeria Enrichment Supplement

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NACRES:
NA.74
UNSPSC Code:
41106212

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agency

APHA, ISO 11133, ISO 11290-1, ISO 11290-2

Quality Level

100

form

lyophilized powder

manufacturer/tradename

ChromoCult®

technique(s)

microbiological culture: suitable

pH

6.6 (20 °C in H2O)

application(s)

food and beverages
microbiology
pathogen testing

compatibility

for use with ChromoCult® Listeria agar (base) acc. OTTAVIANI and AGOSTI (1.00427)

storage temp.

2-8°C

suitability

selective for Listeria spp. (Agar)

相关类别

General description

ChromoCult® Listeria Agar Enrichment-Supplement is a homogeneous, sterile suspension of L-α-phosphatidylinositol in distilled water. Differentiation of Listeria monocytogenes from non-pathogenic Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.

Application

ChromoCult® Listeria Agar Enrichment-Supplement is used as an additive for the preparation of ChromoCult® Listeria Selective Agar (1.00427) for detection and enumeration of Listeria monocytogenes and of Listeria sp. in a variety of food products.

Analysis Note

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 Std. 37°C aerobic; other strains at least 48 hrs.

Other Notes

Replaces: 1004390017

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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商品

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

实验方案

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

Discover the revised EN ISO 11290-1 and EN ISO 11290-2 standards and gain insights into updated detection methods for Listeria monocytogenes and Listeria spp., including enumeration in food, animal feed, and environmental samples. Explore culture media solutions.

Understanding Listeria: Guide to Listeria Monocytogenes and Its Impact on Food Safety

Ensure food safety with our robust Listeria monocytogenes testing methods, including biochemical tests, chromogenic media, and rapid detection methods.

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