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Z374903SIGMA
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引物浓度优化实验方案
引物浓度优化实验方案是一种建立反应阵列的方法。该阵列用来对照伴侣引物的不同浓度来测试每种引物的一系列浓度。
使用SYBR Green染料检测的逆转录实验方案
使用逆转录酶和dNTPs进行逆转录（RT）使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。
标准逆转录方案（两步法）
逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战，例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。
KiCqStart™通用SYBR® Green qPCR实验方案
使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。
选择qPCR参照基因的实验方案
基因表达数据的分析需要稳定的参考或上样控制。所述参考通常是一个或多个参照基因。
使用温度梯度的引物优化试验方案
用于测定优化的梯度PCR通过在一系列退火温度下测试含有固定引物浓度的相同反应物来确定引物的最佳退火温度（Ta）。
qPCR效率测定实验方案
QPCR 条件的优化对于制定稳健的检测方案非常重要。两种主要优化方法是优化引物浓度和/或退火温度。
使用SYBR Green I染料检测的qPCR基因表达/拷贝数分析实验方案
使用 SYBR Green I 染料检测一个或多个稳定内参基因的靶量是qPCR的常见应用。下面是一个可以适应特定实验需要的标准实验方案。
标准PCR实验方案
了解标准PCR方案步骤并查看试剂清单或循环参数。此方法利用标准Taq DNA聚合酶对DNA进行常规PCR扩增。
终点 PCR：特异性和产量增强的抗体介导的热启动 PCR 方案
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
通用SYBR Green qPCR实验方案
我们的SYBR Green qPCR实验方案是一种旨在准确定量基因表达和RT-PCR反应的的方法
Reverse Transcription Protocol (One-step Probe Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
dNTP Hot Start PCR Protocol
Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.
Reverse Transcription Protocol Using SYBR Green Dye Detection
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Multiplex qPCR Protocol
Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.
qPCR with Single Detection Probe
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
Standard Reverse Transcription Protocol
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Primer Concentration Optimization
Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.
Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)
MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.
KiCqStart™ Universal SYBR® Green qPCR Protocol
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Amplification of Genomic DNA using REDTaq® DNA Polymerase
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
qPCR Reference Gene Selection Protocol
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
qPCR Gene Expression Protocol Using SYBR Green
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
Primer Optimization Using Temperature Gradient
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
qPCR Efficiency Determination Protocol
Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.
End Point PCR Protocol for Long and Accurate DNA Amplification
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
The 3'/5' Assay for Analysis of RNA Integrity Protocol
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
SPUD Assay for Detection of Assay Inhibitors Protocol
SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
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