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N3786

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Proteomics Grade, suitable for MALDI-TOF MS

Synonym(s):

Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase

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About This Item

CAS Number:
9001-67-6
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-624-6
MDL number:
MFCD00131711
EC Number:
3.2.1.18 (BRENDA, IUBMB)
Specific activity:
≥25 U/vial
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form

lyophilized powder

Quality Level

200

quality

Proteomics Grade

specific activity

≥25 U/vial

suitability

suitable for MALDI-TOF MS

storage temp.

2-8°C

Application

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Packaging

Provided with 5× concentrate reaction buffer.

Physical form

Lyophilized powder

Analysis Note

The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.

Other Notes

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

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Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • N3536
    Neuraminidase 5X Reaction Buffer 1.5 mL
    SDS

Hazard Classifications

Resp. Sens. 1

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
0000339758
0000339758
SLCJ9368
SLCF3516
SLCC3203

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Articles

Glycan Sequencing Using Exoglycosidases

Glycan Sequencing Using Exoglycosidases

O-Linked Glycan Strategies

Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.

Sialic Acid

Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

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Global Trade Item Number

SKUGTIN
N3786-1SET04061834116301