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Supelco

Micro-Array for Mass Spectrometry (MAMS)

for single cell analysis

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NACRES:
SB.32

surface resistivity

15-25 Ω/sq

technique(s)

MALDI-MS: suitable

L × W × H

75 mm × 25 mm × 1.1 mm

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General description

  • 2x 169 (13x13) spots
  • spot diameter: 100 μm
  • spot depth: ~40 μm
  • pitch: 500 μm

Polysilazane-coated, transparent indium tin oxide target slide, microstructured with an array of 13x13 100 μm diameter spots with a site-to-site distance of 500 μm in both dimensions, produced by picosecond laser ablation. Recipient sites of larger (1.5 mm in diameter) size were machined outside the 13 × 13 array for depositing mass calibrants.

Features and Benefits

MAMS represent a type of substrate for MALDI-MS using a combination of hydrophilic reservoirs surrounded by an omniphobic surface for massively parallel, automated sample spotting. The 13x13 spot array MAMS chip is designed for use in single cell analysis. Cells can easily be distributed in MAMS by pulling a droplet of cell suspension over the microarray surface with a glass slide or piece of plastic resulting in the generation of very reproducible nanoliter aliquots avoiding the need of a robotic microarray spotter.

Compatibility

Compatible with the Bruker Daltonik MTP Slide Adapter II.

Linkage

Spot set designs/geometry files for Bruker MALDI-MS instruments

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Carbon-13 labelling strategy for studying the ATP metabolism in individual yeast cells by micro-arrays for mass spectrometry
Urban, P. L: et al.
Molecular Biosystems, 7, 2837-2840 (2011)
Single-Cell Metabolomics: Analytical and Biological Perspectives
Zenobi, R.
Science, 342, 1201-1201 (2013)
Analysis of single algal cells by combining mass spectrometry with Raman and fluorescence mapping
Fagerer, S. R. et al.
Analyst, 138, 6732-6736 (2013)
Alfredo J Ibáñez et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(22), 8790-8794 (2013-05-15)
Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose)

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