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MSRT2

Sigma-Aldrich

SigmaProt Intact Protein LC-MS Standard

Proteomics Retention Time Standard

Synonym(s):

Intact Protein LC-MS Standard, SigmaProtein Standard

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About This Item

EC Number:
UNSPSC Code:
23201100
NACRES:
NA.24
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Quality Level

form

ready-to-use solution

storage temp.

−20°C

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1 of 4

This Item
GF59105189GF40852728GF49248490
manufacturer/tradename

Goodfellow 085-074-26

manufacturer/tradename

Goodfellow 591-051-89

manufacturer/tradename

Goodfellow 408-527-28

manufacturer/tradename

Goodfellow 492-484-90

form

foil

form

foil

form

foil

form

foil

assay

≥99.6%

assay

≥99.6%

assay

≥99.6%

assay

≥99.6%

bp

3287 °C (lit.)

bp

3287 °C (lit.)

bp

3287 °C (lit.)

bp

3287 °C (lit.)

density

4.5 g/mL at 25 °C (lit.)

density

4.5 g/mL at 25 °C (lit.)

density

4.5 g/mL at 25 °C (lit.)

density

4.5 g/mL at 25 °C (lit.)

resistivity

42.0 μΩ-cm, 20°C

resistivity

42.0 μΩ-cm, 20°C

resistivity

42.0 μΩ-cm, 20°C

resistivity

42.0 μΩ-cm, 20°C

Application

The RP-LC-MS Protein Standard is a LC-MS platform standard that is intended to test LC-MS system suitability and intact protein mass measurement. The proteins in MSRT2 are chosen to represent an elution profile for complex proteomic samples. The proteins have been selected based on UV and electrospray response, and are readily observed by both modes of detection. After reconstitution, MSRT2 is an injection-ready standard. MSRT2 can be utilized in studies such as in top-down proteomics.

Other Notes

MSRT2 contains these 9 proteins:
  • Ribonuclease B
  • Insulin
  • Lysozyme
  • Transferrin
  • Bovine serum albumin (BSA)
  • Trypsin inhibitor
  • β-lactoglobulin A
  • Carbonic anhydrase
  • Lactate dehydrogenase

used together


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    Magdalena Kotańska et al.
    Biochemical pharmacology, 168, 193-203 (2019-07-12)
    Histamine H3 receptors ligands act anorectic by blocking the H3 autoreceptors in the CNS, that results in increased synthesis and disinhibition of histamine release. Histamine further influencing H1 receptors participates in the leptin-dependent inhibition of food intake. It also affects
    Structure-activity relations of histamine analogs. XX. Absolute configuration and histamine-like activity of enantiomeric α-methyl histamines.
    Gerhard, et al.
    Archives of Pharmacal Research, 313, 709-709 (1980)
    J M Arrang et al.
    Nature, 327(6118), 117-123 (1987-05-14)
    New drugs selective for histamine H3-receptors can be used to establish that these receptors are involved in the feedback control of histamine synthesis and release, and to demonstrate their distribution in the brain and peripheral tissues. These drugs provide new
    R Oishi et al.
    Journal of neurochemistry, 52(5), 1388-1392 (1989-05-01)
    To study the feedback control by histamine (HA) H3-receptors on the synthesis and release of HA at nerve endings in the brain, the effects of a potent and selective H3-agonist, (R)-alpha-methylhistamine, and an H3-antagonist, thioperamide, on the pargyline-induced accumulation of
    Xu-Dong Wang et al.
    Nature communications, 10(1), 3201-3201 (2019-07-22)
    Pulmonary neuroendocrine (NE) cancer, including small cell lung cancer (SCLC), is a particularly aggressive malignancy. The lineage-specific transcription factors Achaete-scute homolog 1 (ASCL1), NEUROD1 and POU2F3 have been reported to identify the different subtypes of pulmonary NE cancers. Using a

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