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  • Metabolic engineering for p-coumaryl alcohol production in Escherichia coli by introducing an artificial phenylpropanoid pathway.

Metabolic engineering for p-coumaryl alcohol production in Escherichia coli by introducing an artificial phenylpropanoid pathway.

Biotechnology and applied biochemistry (2014-03-01)
Frank Jansen, Bernhard Gillessen, Frank Mueller, Ulrich Commandeur, Rainer Fischer, Fritz Kreuzaler
ABSTRACT

The plant polymer lignin is the greatest source of aromatic chemical structures on earth. Hence, the chemically diverse lignin monomers are valuable raw materials for fine chemicals, materials synthesis, and food and flavor industries. However, extensive use of this natural resource is hampered by the large number of different lignin monomers and the complex and irregular structure of lignin, which renders current processes for its chemical or enzymatic degradation inefficient. The microbial production of lignin monomers from renewable resources represents a promising alternative to lignin degradation, which could meet the demand for aromatic chemical structures. In this study, we describe the functional introduction of an artificial phenylpropanoid pathway into Escherichia coli, achieved by transferring several genes from plants and microbes. The established chimeric pathway efficiently converts l-tyrosine into the lignin precursor molecule p-coumaryl alcohol.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Tyrosine, FG
Sigma-Aldrich
Sodium trichloroacetate, 97%
Supelco
L-Tyrosine, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
Sigma-Aldrich
Sodium chloride, 99.999% trace metals basis
Sigma-Aldrich
Sodium chloride solution, 0.85%
Imidazole, European Pharmacopoeia (EP) Reference Standard
SAFC
Sodium chloride solution, 5 M
Supelco
L-Tyrosine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
Sigma-Aldrich
Sodium chloride, tested according to Ph. Eur.
Supelco
Sodium chloride, certified reference material for titrimetry, certified by BAM, ≥99.5%
Sigma-Aldrich
L-Tyrosine, BioUltra, ≥99.0% (NT)
Sigma-Aldrich
Imidazole, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Imidazole, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
Imidazole, puriss. p.a., ≥99.5% (GC)
Sigma-Aldrich
p-Coumaric acid, ≥98.0% (HPLC)
Sigma-Aldrich
Sodium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
SAFC
L-Tyrosine
Sigma-Aldrich
Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Sigma-Aldrich
Sodium chloride, tablet
Sigma-Aldrich
Imidazole, ≥99% (titration), crystalline
Sigma-Aldrich
BIS-TRIS propane, BioXtra, ≥99.0% (titration)
Sigma-Aldrich
Nitrilotriacetic acid trisodium salt, Sigma Grade, ≥98%
Sigma-Aldrich
Sodium chloride solution, 5 M
Sigma-Aldrich
BIS-TRIS propane, BioPerformance Certified, suitable for cell culture, ≥99.0%
Sigma-Aldrich
Potassium phosphate tribasic, reagent grade, ≥98%
Sigma-Aldrich
Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
Imidazole, ACS reagent, ≥99% (titration)
Sigma-Aldrich
Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%