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Merck
CN

861049

天青 A 氯化物

certified by the BSC, Dye content 70 %

别名:

Methylene blue

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关于此项目

经验公式(希尔记法):
C14H14ClN3S
化学文摘社编号:
分子量:
291.80
EC Number:
208-510-7
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
52005
Beilstein/REAXYS Number:
3922287
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agency

certified by the BSC

Quality Level

form

powder

composition

Dye content, 70%

color

blue to very dark blue, very dark green

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

λmax

633 nm

ε (extinction coefficient)

≥11000 at 242-246 nm in H2O at -1, ≥26000 at 287-291 nm in H2O, ≥34000 at 620-634 μm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

Application

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.
Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

Biochem/physiol Actions

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels.
van de Lest CH, et al.
Analytical Biochemistry, 221, 356-361 (1994)
C Vindigni et al.
International journal of cancer, 74(6), 637-641 (1998-01-08)
Mitotic (MI) and apoptotic index (AI), the sum of the 2, i.e., the turnover index (TI), tumor neovascularization (NV) and p53 expression, as well as tumor grading and node status, are evaluated in early and advanced gastric-cancer cases. T1 cases
C Priya et al.
Colloids and surfaces. B, Biointerfaces, 97, 90-96 (2012-05-23)
A novel non-enzymatic hydrogen peroxide sensor was developed using Azure A/gold nanoclusters modified graphite electrode. The method of preparation of Azure A/gold nanoclusters was simple and it was characterized by UV-visible spectroscopy, field emission scanning electron microscopy (FESEM) and confocal



全球贸易项目编号

货号GTIN
861049-25G04061833063361
861049-5G04061833040102