assay
>99% (TLC)
form
powder
packaging
pkg of 1 × 1 g (100500P-1g), pkg of 1 × 100 mg (100500P-100mg), pkg of 5 × 100 mg (100500P-500mg)
manufacturer/tradename
Avanti Research™ - A Croda Brand
lipid type
lipid extracts
shipped in
dry ice
storage temp.
−20°C
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General description
该产品是37℃生长于Kornberg Minimal培养基中并在3/4对数生长期提取的大肠杆菌B（ATCC 11303）提取物。总大肠杆菌脂质提取物是各组织的氯仿：甲醇提取物。将该提取液用去离子水进行分配并浓缩氯仿相。
Application
大肠杆菌总提取物已被用于制备单层囊泡，用于基于膜蛋白绿色荧光蛋白（GFP）的融合蛋白的脂质筛选实验以及蛋白脂质体的重组。
Biochem/physiol Actions
大肠杆菌脂质提取物是含有磷脂的16、17和18个碳，即磷脂酰乙醇胺、磷脂酰甘油和心磷脂的一种组合。它可用于制备支持的脂质双层。大肠杆菌脂质提取物在对任何细菌样品进行鉴定和分类时可作为分析磷脂的标准品。
Packaging
5 mL透明玻璃密封安瓿瓶(100500P-100mg)
5 mL透明玻璃密封安瓿瓶(100500P-500mg)
60 mL棕色广口螺帽玻璃瓶(100500P-1g)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins
Nji E, et al.
Nature Communications, 9(1), 4253-4253 (2018)
Membrane insertion of small proteins
Protein Secretion, 39-62 (2010)
Lipid compositions in Escherichia coli and Bacillus subtilis during growth as determined by MALDI-TOF and TOF/TOF mass spectrometry
Gidden J, et al.
International Journal of Mass Spectrometry, 283(1-3), 178-184 (2009)
The extracellular gate shapes the energy profile of an ABC exporter
Hutter CAJ, et al.
Nature Communications, 10(1), 2260-2260 (2019)
Julia Romanov et al.
The EMBO journal, 31(22), 4304-4317 (2012-10-16)
Autophagy is a conserved process for the bulk degradation of cytoplasmic material. Triggering of autophagy results in the formation of double membrane-bound vesicles termed autophagosomes. The conserved Atg5-Atg12/Atg16 complex is essential for autophagosome formation. Here, we show that the yeast
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