05-623-Z
抗RNA聚合酶II抗体，克隆CTD4H8，无腹水
clone CTD4H8, from mouse
别名:
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
产品名称
抗RNA聚合酶II抗体，克隆CTD4H8，无腹水, clone CTD4H8, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CTD4H8, monoclonal
species reactivity
mouse, yeast, rat, human
technique(s)
ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... POLR2A(5430)
mouse ... Polr2A(20020)
rat ... Polr2A(363633)
Analysis Note
通过蛋白质印迹在NIH/3T3细胞裂解液中进行了评估。
蛋白质印迹分析：1 µg/mL该抗体可在10 µg NIH/3T3细胞裂解液中检测到RNA聚合酶II。
蛋白质印迹分析：1 µg/mL该抗体可在10 µg NIH/3T3细胞裂解液中检测到RNA聚合酶II。
Application
抗RNA聚合酶Ⅱ抗体，克隆CTD4H8，无腹水是一种高度特异性的小鼠单克隆抗体，靶向RNA聚合酶，&已在蛋白质印迹，ICC & ChIP-seq中进行了测试。
研究子类别
转录因子
转录因子
研究类别
表观遗传学&核功能
表观遗传学&核功能
蛋白印迹分析：1 µg/mL代表性批次在10 µg HeLa细胞裂解液中检测到RNA聚合酶II。
免疫细胞化学分析：代表性批次的1：50和1：250的稀释液，分别在HeLa和A431细胞中检测到RNA聚合酶II。
免疫细胞化学分析：代表性批次的1：50和1：250的稀释液，分别在HeLa和A431细胞中检测到RNA聚合酶II。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
RNA聚合酶II（Pol II）是负责蛋白质编码基因转录的多亚基酶。转录起始需要通过活化剂和染色质重塑因子的诱导，将完整的转录机制募集到启动子上。Pol II可以协调10至14个亚基。该复合物可与基因的启动子区域以及各种元件和转录因子相互作用。聚合酶的DNA结合结构域是一个TFIIB用于定向DNA以便解链和转录的凹槽。
观测值〜220 kDa
Immunogen
对应于人RNA聚合酶II的线性肽。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
形式：纯化
纯化的小鼠单克隆IgG1κ，溶于含0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl和0.05％叠氮化钠的缓冲液中。（本产品不含腹水。）
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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