产品名称
抗三甲基组蛋白 H3 (Lys4) 抗体，克隆 15-10C-E4，兔单克隆, clone 15-10C-E4, Upstate®, from rabbit
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
15-10C-E4, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
ELISA: suitable
dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
trimethylation (Lys4)
Quality Level
Gene Information
human ... H3F3B(3021)
Analysis Note
Application
代表性批次数据。使用Magna ChIP HiSens试剂盒（目录号17-10460），3 µL 抗三甲基组蛋白H3（Lys4）抗体（目录号05-745R），20 µL蛋白A/G珠和1e6交联 HeLa细胞染色质进行染色质免疫沉淀，然后使用磁珠进行DNA纯化。使用标准规程从 Input 和 ChIP DNA 样品制备文库，带 Illumina 条形码适配器，并在 Illumina HiSeq 仪器上进行分析。在去除 TagDust（http://genome.gsc.riken.jp/osc/english/dataresource）标签后，使用 Bowtie（http://bowtie-bio.sourceforge.net/manual.shtml）映射了 FastQ 文件中的超过1800万次读取。使用 MACS（http://luelab.dfci.harvard.edu/MACS/）识别峰，并从 BigWig 和 BED 文件在 UCSC Genome Browser（http://genome.ucsc.edu）中以自定义轨道的形式显示峰和读数。在05-745R和07-473数据集中识别出的最高25％峰与HeLa S3的ENCODE H3K27Ac BROAD组蛋白轨迹中识别出的峰有99%重叠。
组蛋白
表观遗传学&核功能
Biochem/physiol Actions
Disclaimer
General description
Immunogen
Other Notes
Physical form
Preparation Note
处理建议：收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于 -20°C。避免反复冻融，其可能会破坏IgG并影响产品性能。注意：冷藏室温度变化至低于-20°C时可能导致-含甘油的溶液在储存过程中冻结。
Legal Information
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
相关内容
Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."
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