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Merck
CN

1.04134

曙红Y(淡黄色)(C.I.45380)

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经验公式(希尔记法):
C20H6Br4Na2O5
化学文摘社编号:
分子量:
691.85
UNSPSC Code:
41116130
NACRES:
NA.21
MDL number:
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SMILES string

[Na+].[Na+].Brc1c2[o][c]3[c](c(c2cc([c]1=O)Br)c4c(cccc4)C(=O)[O-])=CC(=C(C=3Br)[O-])Br

InChI

1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

SEACYXSIPDVVMV-UHFFFAOYSA-L

form

solid

potency

>2000 mg/kg LD50, oral (Rat)

composition

Dye, ≥85% spectrophotometric assay

pH

9.2 (20 °C, 10 g/L in H2O)

mp

295-296 °C

solubility

300 g/L

bulk density

710 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

Quality Level

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Analysis Note

Dye content (gravimetrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 515 - 518 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 1200 - 1400
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Application

for microscopy Certistain®

General description

Eosin Y (C.I. 45380) is a CERTISTAIN® dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin Y staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange. As the Eosin Y dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release and a high staining quality, with reliable staining results is guaranteed.

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

376.9 °F - Pensky-Martens closed cup

flash_point_c

191.6 °C - Pensky-Martens closed cup

法规信息

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