Merck
CN

1.04445

SAFC

Benzonase® Salt Tolerant Endonuclease

newgreener alternative

EMPROVE® EXPERT

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产品线

EMPROVE® EXPERT

质量水平

环保替代产品特性

Waste Prevention
Learn more about the Principles of Green Chemistry.

应用

pharma/biopharma processes

环保替代产品分类

储存温度

−20°C

相关类别

一般描述

The best-in-class solution for nucleic acid removal at high salt concentration in Bioprocessing
We are committed to bringing you Greener AlternativeProducts, which belongs to one of the four categories of greener alternatives .This product is a Design for Sustainability (DfS) developed product. Click hereto view its DfS scorecard.

应用

The Benzonase® Salt Tolerant endonuclease Emprove® Expert is a new enzyme, different in amino acids sequence from other Benzonase® endonuclease references. It was designed with state-of-the-art protein engineering capabilities, to ensure the highest activity at high salt concentrations. The protein is a monomer with a molecular weight of about 27 kDa . The protein has an isoelectric point (pI) at pH 9.68. It is functional between pH 6 and 10, and from 0 °C to above 37 °C. Mg2+ (1–2 mM) is required for enzyme activity.

包装

  • 80,000 Units
  • 400,000 Units
  • 4,000,000 Units + 40, 000 Units tailgate sample

法律信息

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 1

法规信息

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商品

本文介绍了细胞裂解的主要考虑因素,以及如何通过高盐浓度配合耐高盐核酸内切酶提高AAV载体生产工艺的病毒滴度和感染能力。

This page describes key considerations for cell lysis and how the combination of a high salt concentration and a salt tolerant endonuclease can be used to increase vector titer and infectivity during AAV vector manufacturing.

Whether you are developing cell and gene therapies or viral vaccines, removing contaminating nucleic acids is an important part of viral production. The simplest way to do this is to use the Benzonase® endonuclease.

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