product line
EMPROVE® EXPERT
greener alternative product characteristics
Waste Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
application(s)
pharma/biopharma processes
greener alternative category
storage temp.
-10 to -25°C
Quality Level
General description
The best-in-class solution for nucleic acid removal at high salt concentration in Bioprocessing
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Application
The Benzonase® Salt Tolerant endonuclease Emprove® Expert is a new enzyme, different in amino acids sequence from other Benzonase® endonuclease references. It was designed with state-of-the-art protein engineering capabilities, to ensure the highest activity at high salt concentrations. The protein is a monomer with a molecular weight of about 27 kDa . The protein has an isoelectric point (pI) at pH 9.68. It is functional between pH 6 and 10, and from 0 °C to above 37 °C. Mg2+ (1–2 mM) is required for enzyme activity.
Packaging
- 80,000 Units
- 400,000 Units
- 4,000,000 Units + 40, 000 Units tailgate sample
Legal Information
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 1
法规信息
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商品
This page describes key considerations for cell lysis and how the combination of a high salt concentration and a salt tolerant endonuclease can be used to increase vector titer and infectivity during AAV vector manufacturing.
Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.
本文介绍了细胞裂解的主要考虑因素,以及如何通过高盐浓度配合耐高盐核酸内切酶提高AAV载体生产工艺的病毒滴度和感染能力。
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